Experiment: Cephalothin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0644 and Shew_0645 are separated by 9 nucleotides Shew_0645 and Shew_0646 overlap by 4 nucleotides Shew_0646 and Shew_0647 are separated by 14 nucleotides
Shew_0644: Shew_0644 - FHA domain-containing protein (RefSeq), at 732,169 to 733,878
_0644
Shew_0645: Shew_0645 - type II and III secretion system protein (RefSeq), at 733,888 to 735,198
_0645
Shew_0646: Shew_0646 - SAF domain-containing protein (RefSeq), at 735,195 to 736,091
_0646
Shew_0647: Shew_0647 - hypothetical protein (RefSeq), at 736,106 to 736,297
_0647
Position (kb) 733
734
735
736 Strain fitness (log2 ratio) -2
-1
0
1
2 at 733.265 kb on - strand, within Shew_0644 at 733.371 kb on + strand, within Shew_0644 at 733.469 kb on + strand, within Shew_0644 at 733.541 kb on - strand, within Shew_0644 at 733.884 kb on + strand at 733.987 kb on - strand at 733.987 kb on - strand at 734.132 kb on - strand, within Shew_0645 at 734.132 kb on - strand, within Shew_0645 at 734.690 kb on + strand, within Shew_0645 at 734.931 kb on + strand, within Shew_0645 at 735.233 kb on - strand at 735.270 kb on + strand at 735.539 kb on + strand, within Shew_0646 at 735.875 kb on - strand, within Shew_0646 at 735.875 kb on - strand, within Shew_0646 at 735.952 kb on + strand, within Shew_0646 at 736.189 kb on - strand, within Shew_0647
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.1 mg/ml remove 733,265 - Shew_0644 0.64 +1.4 733,371 + Shew_0644 0.70 -0.6 733,469 + Shew_0644 0.76 +0.0 733,541 - Shew_0644 0.80 +0.9 733,884 + +0.1 733,987 - -0.0 733,987 - +0.8 734,132 - Shew_0645 0.19 +1.9 734,132 - Shew_0645 0.19 +0.5 734,690 + Shew_0645 0.61 -0.5 734,931 + Shew_0645 0.80 -0.3 735,233 - +0.4 735,270 + +0.7 735,539 + Shew_0646 0.38 +0.2 735,875 - Shew_0646 0.76 +0.3 735,875 - Shew_0646 0.76 -2.5 735,952 + Shew_0646 0.84 +1.0 736,189 - Shew_0647 0.43 +0.5
Or see this region's nucleotide sequence