Experiment: Cephalothin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0305 and Shew_0306 are separated by 29 nucleotides Shew_0306 and Shew_R0014 are separated by 218 nucleotides Shew_R0014 and Shew_R0015 are separated by 43 nucleotides Shew_R0015 and Shew_R0016 are separated by 84 nucleotides Shew_R0016 and Shew_R0017 are separated by 52 nucleotides Shew_R0017 and Shew_0307 are separated by 132 nucleotides
Shew_0305: Shew_0305 - ATP-dependent DNA helicase Rep (RefSeq), at 327,074 to 329,086
_0305
Shew_0306: Shew_0306 - diguanylate cyclase/phosphodiesterase with GAF sensor (RefSeq), at 329,116 to 331,764
_0306
Shew_R0014: Shew_R0014 - tRNA-Arg (RefSeq), at 331,983 to 332,059
_R0014
Shew_R0015: Shew_R0015 - tRNA-His (RefSeq), at 332,103 to 332,178
_R0015
Shew_R0016: Shew_R0016 - tRNA-Pro (RefSeq), at 332,263 to 332,339
_R0016
Shew_R0017: Shew_R0017 - tRNA-Pro (RefSeq), at 332,392 to 332,468
_R0017
Shew_0307: Shew_0307 - diguanylate cyclase/phosphodiesterase (RefSeq), at 332,601 to 334,523
_0307
Position (kb)
329
330
331
332 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 329.254 kb on - strand at 329.395 kb on + strand, within Shew_0306 at 329.395 kb on + strand, within Shew_0306 at 329.395 kb on + strand, within Shew_0306 at 329.395 kb on + strand, within Shew_0306 at 329.395 kb on + strand, within Shew_0306 at 329.403 kb on - strand, within Shew_0306 at 329.430 kb on + strand, within Shew_0306 at 329.430 kb on + strand, within Shew_0306 at 329.438 kb on - strand, within Shew_0306 at 329.438 kb on - strand, within Shew_0306 at 329.438 kb on - strand, within Shew_0306 at 329.438 kb on - strand, within Shew_0306 at 329.548 kb on - strand, within Shew_0306 at 329.625 kb on + strand, within Shew_0306 at 329.674 kb on - strand, within Shew_0306 at 329.674 kb on - strand, within Shew_0306 at 329.948 kb on + strand, within Shew_0306 at 330.369 kb on + strand, within Shew_0306 at 330.536 kb on - strand, within Shew_0306 at 330.536 kb on - strand, within Shew_0306 at 330.650 kb on - strand, within Shew_0306 at 330.749 kb on + strand, within Shew_0306 at 330.772 kb on - strand, within Shew_0306 at 330.935 kb on + strand, within Shew_0306 at 331.026 kb on - strand, within Shew_0306 at 331.080 kb on + strand, within Shew_0306 at 331.084 kb on - strand, within Shew_0306 at 331.094 kb on - strand, within Shew_0306 at 331.099 kb on - strand, within Shew_0306 at 331.164 kb on - strand, within Shew_0306 at 331.176 kb on + strand, within Shew_0306 at 331.407 kb on - strand, within Shew_0306 at 331.484 kb on - strand, within Shew_0306 at 331.986 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.1 mg/ml remove 329,254 - +0.2 329,395 + Shew_0306 0.11 -0.5 329,395 + Shew_0306 0.11 -0.2 329,395 + Shew_0306 0.11 -0.2 329,395 + Shew_0306 0.11 -2.1 329,395 + Shew_0306 0.11 +0.1 329,403 - Shew_0306 0.11 +0.6 329,430 + Shew_0306 0.12 +0.2 329,430 + Shew_0306 0.12 -0.6 329,438 - Shew_0306 0.12 +1.8 329,438 - Shew_0306 0.12 -0.6 329,438 - Shew_0306 0.12 -0.1 329,438 - Shew_0306 0.12 +0.9 329,548 - Shew_0306 0.16 -0.1 329,625 + Shew_0306 0.19 -1.4 329,674 - Shew_0306 0.21 +0.1 329,674 - Shew_0306 0.21 +0.3 329,948 + Shew_0306 0.31 -0.8 330,369 + Shew_0306 0.47 -0.4 330,536 - Shew_0306 0.54 -0.2 330,536 - Shew_0306 0.54 +0.8 330,650 - Shew_0306 0.58 -0.3 330,749 + Shew_0306 0.62 +0.1 330,772 - Shew_0306 0.63 +0.6 330,935 + Shew_0306 0.69 +0.1 331,026 - Shew_0306 0.72 -0.5 331,080 + Shew_0306 0.74 -0.2 331,084 - Shew_0306 0.74 +0.4 331,094 - Shew_0306 0.75 -1.3 331,099 - Shew_0306 0.75 +0.9 331,164 - Shew_0306 0.77 +0.4 331,176 + Shew_0306 0.78 +0.0 331,407 - Shew_0306 0.86 +0.6 331,484 - Shew_0306 0.89 -0.3 331,986 + -1.4
Or see this region's nucleotide sequence