Experiment: Cephalothin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0026 and Shew_0027 are separated by 12 nucleotides Shew_0027 and Shew_0028 are separated by 81 nucleotides Shew_0028 and Shew_0029 are separated by 229 nucleotides
Shew_0026: Shew_0026 - hypothetical protein (RefSeq), at 28,832 to 29,368
_0026
Shew_0027: Shew_0027 - glutathione S-transferase domain-containing protein (RefSeq), at 29,381 to 30,028
_0027
Shew_0028: Shew_0028 - LysR family transcriptional regulator (RefSeq), at 30,110 to 31,009
_0028
Shew_0029: Shew_0029 - 3-methyladenine DNA glycosylase (RefSeq), at 31,239 to 31,928
_0029
Position (kb)
30
31
32 Strain fitness (log2 ratio)
-1
0
1 at 29.135 kb on + strand, within Shew_0026 at 29.328 kb on - strand at 29.371 kb on - strand at 29.774 kb on - strand, within Shew_0027 at 29.889 kb on + strand, within Shew_0027 at 30.115 kb on - strand at 30.128 kb on - strand at 30.324 kb on - strand, within Shew_0028 at 30.531 kb on + strand, within Shew_0028 at 30.539 kb on - strand, within Shew_0028 at 30.559 kb on - strand, within Shew_0028 at 30.572 kb on - strand, within Shew_0028 at 30.688 kb on - strand, within Shew_0028 at 30.780 kb on + strand, within Shew_0028 at 30.790 kb on + strand, within Shew_0028 at 30.951 kb on - strand at 31.272 kb on - strand at 31.658 kb on + strand, within Shew_0029 at 31.658 kb on + strand, within Shew_0029 at 31.658 kb on + strand, within Shew_0029 at 31.658 kb on + strand, within Shew_0029 at 31.666 kb on - strand, within Shew_0029 at 31.893 kb on + strand at 31.908 kb on - strand at 31.936 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.1 mg/ml remove 29,135 + Shew_0026 0.56 +0.1 29,328 - -0.8 29,371 - +0.1 29,774 - Shew_0027 0.61 -0.4 29,889 + Shew_0027 0.78 -0.3 30,115 - +1.3 30,128 - -0.7 30,324 - Shew_0028 0.24 -1.0 30,531 + Shew_0028 0.47 -0.1 30,539 - Shew_0028 0.48 -1.4 30,559 - Shew_0028 0.50 +0.6 30,572 - Shew_0028 0.51 -0.4 30,688 - Shew_0028 0.64 -1.2 30,780 + Shew_0028 0.74 -0.1 30,790 + Shew_0028 0.76 +1.1 30,951 - +0.1 31,272 - -0.7 31,658 + Shew_0029 0.61 -1.1 31,658 + Shew_0029 0.61 +0.2 31,658 + Shew_0029 0.61 -0.3 31,658 + Shew_0029 0.61 +0.1 31,666 - Shew_0029 0.62 +0.0 31,893 + -0.0 31,908 - -0.4 31,936 - -0.1
Or see this region's nucleotide sequence