Experiment: Paraquat dichloride 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpoN and yfiA-3 are separated by 28 nucleotides yfiA-3 and ptsN are separated by 2 nucleotides ptsN and SO3964 overlap by 4 nucleotides
SO3961: rpoN - RNA polymerase sigma-54 factor (NCBI ptt file), at 4,102,103 to 4,103,578
rpoN
SO3962: yfiA-3 - ribosomal subunit interface protein (NCBI ptt file), at 4,103,607 to 4,103,894
yfiA-3
SO3963: ptsN - nitrogen regulatory IIA protein (NCBI ptt file), at 4,103,897 to 4,104,340
ptsN
SO3964: SO3964 - conserved hypothetical protein (NCBI ptt file), at 4,104,337 to 4,105,191
SO3964
Position (kb)
4103
4104 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4102.645 kb on - strand, within rpoN at 4102.648 kb on + strand, within rpoN at 4102.678 kb on - strand, within rpoN at 4102.901 kb on - strand, within rpoN at 4102.936 kb on - strand, within rpoN at 4103.142 kb on + strand, within rpoN at 4103.157 kb on + strand, within rpoN at 4103.165 kb on - strand, within rpoN at 4103.197 kb on - strand, within rpoN at 4103.365 kb on + strand, within rpoN at 4103.365 kb on + strand, within rpoN at 4103.373 kb on - strand, within rpoN at 4103.373 kb on - strand, within rpoN at 4103.383 kb on - strand, within rpoN at 4103.389 kb on + strand, within rpoN at 4103.389 kb on + strand, within rpoN at 4103.397 kb on - strand, within rpoN at 4103.487 kb on - strand at 4103.535 kb on - strand at 4103.561 kb on - strand at 4103.563 kb on + strand at 4103.563 kb on + strand at 4103.642 kb on - strand, within yfiA-3 at 4103.657 kb on + strand, within yfiA-3 at 4103.705 kb on + strand, within yfiA-3 at 4103.823 kb on + strand, within yfiA-3 at 4103.895 kb on + strand at 4103.942 kb on + strand, within ptsN at 4103.942 kb on + strand, within ptsN at 4103.950 kb on - strand, within ptsN at 4103.993 kb on + strand, within ptsN at 4104.001 kb on - strand, within ptsN at 4104.065 kb on + strand, within ptsN at 4104.065 kb on + strand, within ptsN at 4104.108 kb on + strand, within ptsN at 4104.278 kb on + strand, within ptsN at 4104.386 kb on + strand at 4104.394 kb on - strand at 4104.394 kb on - strand at 4104.465 kb on + strand, within SO3964 at 4104.473 kb on - strand, within SO3964 at 4104.473 kb on - strand, within SO3964 at 4104.574 kb on - strand, within SO3964
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.125 mg/ml remove 4,102,645 - rpoN SO3961 0.37 -0.7 4,102,648 + rpoN SO3961 0.37 -1.0 4,102,678 - rpoN SO3961 0.39 -0.5 4,102,901 - rpoN SO3961 0.54 -0.3 4,102,936 - rpoN SO3961 0.56 -0.2 4,103,142 + rpoN SO3961 0.70 -1.3 4,103,157 + rpoN SO3961 0.71 +0.2 4,103,165 - rpoN SO3961 0.72 -0.9 4,103,197 - rpoN SO3961 0.74 -0.7 4,103,365 + rpoN SO3961 0.86 -0.5 4,103,365 + rpoN SO3961 0.86 -1.2 4,103,373 - rpoN SO3961 0.86 -0.2 4,103,373 - rpoN SO3961 0.86 -2.7 4,103,383 - rpoN SO3961 0.87 -1.4 4,103,389 + rpoN SO3961 0.87 -1.7 4,103,389 + rpoN SO3961 0.87 +0.3 4,103,397 - rpoN SO3961 0.88 -0.6 4,103,487 - -1.7 4,103,535 - +0.0 4,103,561 - -0.8 4,103,563 + -0.1 4,103,563 + +0.4 4,103,642 - yfiA-3 SO3962 0.12 -0.8 4,103,657 + yfiA-3 SO3962 0.17 -0.3 4,103,705 + yfiA-3 SO3962 0.34 -0.9 4,103,823 + yfiA-3 SO3962 0.75 -0.8 4,103,895 + +0.1 4,103,942 + ptsN SO3963 0.10 -2.7 4,103,942 + ptsN SO3963 0.10 -3.0 4,103,950 - ptsN SO3963 0.12 -1.8 4,103,993 + ptsN SO3963 0.22 -3.6 4,104,001 - ptsN SO3963 0.23 -2.8 4,104,065 + ptsN SO3963 0.38 -1.5 4,104,065 + ptsN SO3963 0.38 -2.2 4,104,108 + ptsN SO3963 0.48 -3.6 4,104,278 + ptsN SO3963 0.86 -2.6 4,104,386 + +0.6 4,104,394 - -0.1 4,104,394 - -0.2 4,104,465 + SO3964 0.15 +0.8 4,104,473 - SO3964 0.16 +0.1 4,104,473 - SO3964 0.16 +0.2 4,104,574 - SO3964 0.28 +0.3
Or see this region's nucleotide sequence