Experiment: Paraquat dichloride 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3854 and sfcA are separated by 341 nucleotides sfcA and SO3856 are separated by 73 nucleotides SO3856 and SO3857 are separated by 23 nucleotides
SO3854: SO3854 - ISSod12, transposase (NCBI ptt file), at 3,999,523 to 4,000,446
SO3854
SO3855: sfcA - malate oxidoreductase (NCBI ptt file), at 4,000,788 to 4,002,476
sfcA
SO3856: SO3856 - hypothetical protein (NCBI ptt file), at 4,002,550 to 4,002,984
SO3856
SO3857: SO3857 - PAP2 family protein (NCBI ptt file), at 4,003,008 to 4,003,535
SO3857
Position (kb)
4000
4001
4002
4003 Strain fitness (log2 ratio)
-2
-1
0
1 at 3999.803 kb on - strand, within SO3854 at 3999.803 kb on - strand, within SO3854 at 3999.863 kb on + strand, within SO3854 at 3999.865 kb on + strand, within SO3854 at 3999.865 kb on + strand, within SO3854 at 3999.871 kb on - strand, within SO3854 at 3999.871 kb on - strand, within SO3854 at 3999.885 kb on - strand, within SO3854 at 3999.921 kb on - strand, within SO3854 at 4000.234 kb on - strand, within SO3854 at 4000.326 kb on + strand, within SO3854 at 4000.411 kb on + strand at 4000.411 kb on + strand at 4000.411 kb on + strand at 4000.420 kb on - strand at 4000.430 kb on - strand at 4000.460 kb on + strand at 4000.551 kb on + strand at 4000.730 kb on + strand at 4000.749 kb on - strand at 4000.776 kb on - strand at 4000.781 kb on - strand at 4000.908 kb on + strand at 4000.924 kb on - strand at 4000.946 kb on + strand at 4000.965 kb on - strand, within sfcA at 4001.022 kb on - strand, within sfcA at 4001.045 kb on - strand, within sfcA at 4001.100 kb on - strand, within sfcA at 4001.138 kb on + strand, within sfcA at 4001.153 kb on + strand, within sfcA at 4001.325 kb on + strand, within sfcA at 4001.330 kb on + strand, within sfcA at 4001.333 kb on - strand, within sfcA at 4001.359 kb on + strand, within sfcA at 4001.359 kb on + strand, within sfcA at 4001.619 kb on - strand, within sfcA at 4001.647 kb on + strand, within sfcA at 4001.655 kb on - strand, within sfcA at 4001.685 kb on - strand, within sfcA at 4001.705 kb on - strand, within sfcA at 4001.812 kb on + strand, within sfcA at 4001.939 kb on - strand, within sfcA at 4001.939 kb on - strand, within sfcA at 4001.953 kb on + strand, within sfcA at 4001.953 kb on + strand, within sfcA at 4001.961 kb on - strand, within sfcA at 4001.996 kb on - strand, within sfcA at 4002.090 kb on + strand, within sfcA at 4002.158 kb on + strand, within sfcA at 4002.176 kb on - strand, within sfcA at 4002.197 kb on - strand, within sfcA at 4002.243 kb on + strand, within sfcA at 4002.294 kb on + strand, within sfcA at 4002.378 kb on + strand at 4002.378 kb on + strand at 4002.574 kb on + strand at 4002.585 kb on - strand at 4002.635 kb on - strand, within SO3856 at 4002.635 kb on - strand, within SO3856 at 4002.635 kb on - strand, within SO3856 at 4002.770 kb on + strand, within SO3856 at 4002.778 kb on - strand, within SO3856 at 4002.808 kb on - strand, within SO3856 at 4002.813 kb on - strand, within SO3856 at 4002.813 kb on - strand, within SO3856 at 4002.863 kb on - strand, within SO3856 at 4002.961 kb on - strand at 4003.099 kb on + strand, within SO3857 at 4003.167 kb on + strand, within SO3857 at 4003.247 kb on + strand, within SO3857 at 4003.256 kb on + strand, within SO3857 at 4003.276 kb on - strand, within SO3857 at 4003.325 kb on + strand, within SO3857 at 4003.333 kb on - strand, within SO3857 at 4003.333 kb on - strand, within SO3857 at 4003.334 kb on - strand, within SO3857 at 4003.350 kb on + strand, within SO3857 at 4003.358 kb on + strand, within SO3857 at 4003.358 kb on - strand, within SO3857 at 4003.368 kb on + strand, within SO3857 at 4003.391 kb on - strand, within SO3857 at 4003.440 kb on - strand, within SO3857
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.125 mg/ml remove 3,999,803 - SO3854 0.30 -0.6 3,999,803 - SO3854 0.30 +0.9 3,999,863 + SO3854 0.37 +0.1 3,999,865 + SO3854 0.37 +0.2 3,999,865 + SO3854 0.37 -0.2 3,999,871 - SO3854 0.38 -0.8 3,999,871 - SO3854 0.38 +0.2 3,999,885 - SO3854 0.39 +0.3 3,999,921 - SO3854 0.43 +0.5 4,000,234 - SO3854 0.77 -0.7 4,000,326 + SO3854 0.87 +0.1 4,000,411 + -0.2 4,000,411 + +0.3 4,000,411 + +0.3 4,000,420 - -0.6 4,000,430 - +0.0 4,000,460 + +0.1 4,000,551 + -0.4 4,000,730 + -0.2 4,000,749 - -0.3 4,000,776 - -1.1 4,000,781 - -0.0 4,000,908 + -0.8 4,000,924 - -0.9 4,000,946 + -0.3 4,000,965 - sfcA SO3855 0.10 -0.3 4,001,022 - sfcA SO3855 0.14 -1.6 4,001,045 - sfcA SO3855 0.15 -0.2 4,001,100 - sfcA SO3855 0.18 -1.0 4,001,138 + sfcA SO3855 0.21 -1.7 4,001,153 + sfcA SO3855 0.22 +0.1 4,001,325 + sfcA SO3855 0.32 -1.0 4,001,330 + sfcA SO3855 0.32 -0.0 4,001,333 - sfcA SO3855 0.32 -1.2 4,001,359 + sfcA SO3855 0.34 -0.7 4,001,359 + sfcA SO3855 0.34 -0.5 4,001,619 - sfcA SO3855 0.49 -1.3 4,001,647 + sfcA SO3855 0.51 -0.5 4,001,655 - sfcA SO3855 0.51 -1.1 4,001,685 - sfcA SO3855 0.53 -1.1 4,001,705 - sfcA SO3855 0.54 -0.7 4,001,812 + sfcA SO3855 0.61 -0.7 4,001,939 - sfcA SO3855 0.68 -1.6 4,001,939 - sfcA SO3855 0.68 +0.2 4,001,953 + sfcA SO3855 0.69 -0.3 4,001,953 + sfcA SO3855 0.69 -0.6 4,001,961 - sfcA SO3855 0.69 +1.0 4,001,996 - sfcA SO3855 0.72 -0.5 4,002,090 + sfcA SO3855 0.77 -0.4 4,002,158 + sfcA SO3855 0.81 -0.9 4,002,176 - sfcA SO3855 0.82 -2.1 4,002,197 - sfcA SO3855 0.83 -0.5 4,002,243 + sfcA SO3855 0.86 -0.8 4,002,294 + sfcA SO3855 0.89 -0.7 4,002,378 + -2.1 4,002,378 + -1.0 4,002,574 + -0.8 4,002,585 - -0.2 4,002,635 - SO3856 0.20 +0.5 4,002,635 - SO3856 0.20 -0.2 4,002,635 - SO3856 0.20 -0.2 4,002,770 + SO3856 0.51 -0.3 4,002,778 - SO3856 0.52 +0.2 4,002,808 - SO3856 0.59 +0.3 4,002,813 - SO3856 0.60 -0.1 4,002,813 - SO3856 0.60 +0.4 4,002,863 - SO3856 0.72 +1.0 4,002,961 - -0.5 4,003,099 + SO3857 0.17 -0.1 4,003,167 + SO3857 0.30 -0.3 4,003,247 + SO3857 0.45 -0.2 4,003,256 + SO3857 0.47 -0.2 4,003,276 - SO3857 0.51 +0.4 4,003,325 + SO3857 0.60 +0.2 4,003,333 - SO3857 0.62 -0.2 4,003,333 - SO3857 0.62 +0.1 4,003,334 - SO3857 0.62 -0.2 4,003,350 + SO3857 0.65 -0.1 4,003,358 + SO3857 0.66 +0.5 4,003,358 - SO3857 0.66 -0.0 4,003,368 + SO3857 0.68 +0.5 4,003,391 - SO3857 0.73 +0.1 4,003,440 - SO3857 0.82 -0.2
Or see this region's nucleotide sequence