Experiment: Paraquat dichloride 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mtrD and feoA are separated by 470 nucleotides feoA and feoB overlap by 8 nucleotides feoB and glnS are separated by 184 nucleotides
SO1782: mtrD - decaheme cytochrome c MtrD (NCBI ptt file), at 1,868,251 to 1,869,171
mtrD
SO1783: feoA - ferrous iron transport protein A (NCBI ptt file), at 1,869,642 to 1,869,881
feoA
SO1784: feoB - ferrous iron transport protein B (NCBI ptt file), at 1,869,874 to 1,872,168
feoB
SO1786: glnS - glutaminyl-tRNA synthetase (NCBI ptt file), at 1,872,353 to 1,874,023
glnS
Position (kb)
1869
1870
1871
1872
1873 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1869.063 kb on - strand, within mtrD at 1869.127 kb on + strand at 1869.133 kb on + strand at 1869.141 kb on - strand at 1869.368 kb on - strand at 1869.405 kb on + strand at 1869.405 kb on + strand at 1869.424 kb on + strand at 1869.426 kb on + strand at 1869.426 kb on + strand at 1869.510 kb on + strand at 1869.587 kb on + strand at 1869.597 kb on - strand at 1869.860 kb on + strand at 1872.125 kb on + strand at 1872.252 kb on + strand at 1872.252 kb on + strand at 1872.252 kb on + strand at 1872.260 kb on - strand at 1872.260 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.125 mg/ml remove 1,869,063 - mtrD SO1782 0.88 -0.3 1,869,127 + +1.0 1,869,133 + +1.2 1,869,141 - +0.1 1,869,368 - +0.8 1,869,405 + -0.1 1,869,405 + +0.2 1,869,424 + -0.0 1,869,426 + -0.1 1,869,426 + -0.1 1,869,510 + +0.2 1,869,587 + -0.7 1,869,597 - +0.1 1,869,860 + +0.2 1,872,125 + -3.0 1,872,252 + -0.2 1,872,252 + +0.3 1,872,252 + +0.1 1,872,260 - -0.3 1,872,260 - -0.0
Or see this region's nucleotide sequence