Experiment: Paraquat dichloride 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hflC and petA are separated by 360 nucleotides petA and petB overlap by 1 nucleotides petB and petC overlap by 1 nucleotides petC and sspA are separated by 96 nucleotides
SO0606: hflC - hflC protein (NCBI ptt file), at 632,545 to 633,438
hflC
SO0608: petA - ubiquinol-cytochrome c reductase, iron-sulfur subunit (NCBI ptt file), at 633,799 to 634,389
petA
SO0609: petB - ubiquinol-cytochrome c reductase, cytochrome b (NCBI ptt file), at 634,389 to 635,603
petB
SO0610: petC - ubiquinol-cytochrome c reductase, cytochrome c1 (NCBI ptt file), at 635,603 to 636,298
petC
SO0611: sspA - stringent starvation protein a (NCBI ptt file), at 636,395 to 637,024
sspA
Position (kb) 634
635
636 Strain fitness (log2 ratio) -2
-1
0
1 at 633.845 kb on - strand at 634.379 kb on - strand at 634.631 kb on - strand, within petB at 634.631 kb on - strand, within petB at 634.717 kb on - strand, within petB at 634.929 kb on + strand, within petB at 634.983 kb on + strand, within petB at 635.012 kb on + strand, within petB at 635.032 kb on + strand, within petB at 635.123 kb on - strand, within petB at 635.190 kb on + strand, within petB at 635.222 kb on + strand, within petB at 635.239 kb on + strand, within petB at 635.242 kb on - strand, within petB at 635.360 kb on + strand, within petB at 635.360 kb on + strand, within petB at 635.701 kb on + strand, within petC at 636.333 kb on + strand at 636.346 kb on + strand at 636.500 kb on - strand, within sspA at 636.584 kb on - strand, within sspA
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.125 mg/ml remove 633,845 - -0.2 634,379 - -1.5 634,631 - petB SO0609 0.20 -0.7 634,631 - petB SO0609 0.20 -1.9 634,717 - petB SO0609 0.27 -0.9 634,929 + petB SO0609 0.44 -0.5 634,983 + petB SO0609 0.49 -1.6 635,012 + petB SO0609 0.51 -2.7 635,032 + petB SO0609 0.53 -0.8 635,123 - petB SO0609 0.60 -0.3 635,190 + petB SO0609 0.66 -2.2 635,222 + petB SO0609 0.69 -0.6 635,239 + petB SO0609 0.70 -0.8 635,242 - petB SO0609 0.70 -1.1 635,360 + petB SO0609 0.80 -0.8 635,360 + petB SO0609 0.80 -0.3 635,701 + petC SO0610 0.14 -0.9 636,333 + -0.2 636,346 + +0.2 636,500 - sspA SO0611 0.17 -0.5 636,584 - sspA SO0611 0.30 -1.3
Or see this region's nucleotide sequence