Experiment: Paraquat dichloride 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0342 and acnA are separated by 86 nucleotides acnA and prpC are separated by 80 nucleotides
SO0342: SO0342 - conserved hypothetical protein (NCBI ptt file), at 346,264 to 347,457
SO0342
SO0343: acnA - aconitate hydratase 1 (NCBI ptt file), at 347,544 to 350,147
acnA
SO0344: prpC - 2-methylcitrate synthase (EC 2.3.3.5) <small>(from data)</small>, at 350,228 to 351,355
prpC
Position (kb) 347
348
349
350
351 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 346.670 kb on - strand, within SO0342 at 346.670 kb on - strand, within SO0342 at 346.702 kb on - strand, within SO0342 at 346.710 kb on + strand, within SO0342 at 346.743 kb on + strand, within SO0342 at 346.743 kb on + strand, within SO0342 at 346.743 kb on + strand, within SO0342 at 346.751 kb on - strand, within SO0342 at 346.935 kb on - strand, within SO0342 at 347.051 kb on + strand, within SO0342 at 347.148 kb on - strand, within SO0342 at 347.184 kb on - strand, within SO0342 at 347.237 kb on - strand, within SO0342 at 347.285 kb on + strand, within SO0342 at 347.297 kb on + strand, within SO0342 at 347.354 kb on + strand at 347.362 kb on - strand at 347.362 kb on - strand at 347.428 kb on + strand at 347.649 kb on - strand at 347.696 kb on - strand at 347.740 kb on - strand at 347.795 kb on + strand at 347.876 kb on - strand, within acnA at 347.889 kb on + strand, within acnA at 347.897 kb on - strand, within acnA at 347.901 kb on + strand, within acnA at 347.907 kb on + strand, within acnA at 347.909 kb on - strand, within acnA at 347.951 kb on + strand, within acnA at 347.980 kb on + strand, within acnA at 348.102 kb on - strand, within acnA at 348.231 kb on + strand, within acnA at 348.343 kb on + strand, within acnA at 348.407 kb on + strand, within acnA at 348.412 kb on + strand, within acnA at 348.412 kb on + strand, within acnA at 348.415 kb on - strand, within acnA at 348.420 kb on - strand, within acnA at 348.420 kb on - strand, within acnA at 348.420 kb on - strand, within acnA at 348.420 kb on - strand, within acnA at 348.557 kb on + strand, within acnA at 348.600 kb on - strand, within acnA at 348.600 kb on - strand, within acnA at 348.600 kb on - strand, within acnA at 348.707 kb on - strand, within acnA at 348.873 kb on + strand, within acnA at 348.891 kb on + strand, within acnA at 348.944 kb on - strand, within acnA at 348.965 kb on - strand, within acnA at 349.015 kb on - strand, within acnA at 349.015 kb on - strand, within acnA at 349.048 kb on + strand, within acnA at 349.048 kb on + strand, within acnA at 349.063 kb on + strand, within acnA at 349.071 kb on - strand, within acnA at 349.087 kb on + strand, within acnA at 349.122 kb on + strand, within acnA at 349.164 kb on - strand, within acnA at 349.164 kb on - strand, within acnA at 349.172 kb on - strand, within acnA at 349.195 kb on - strand, within acnA at 349.224 kb on - strand, within acnA at 349.224 kb on - strand, within acnA at 349.224 kb on - strand, within acnA at 349.291 kb on - strand, within acnA at 349.420 kb on - strand, within acnA at 349.460 kb on + strand, within acnA at 349.460 kb on + strand, within acnA at 349.534 kb on - strand, within acnA at 349.540 kb on + strand, within acnA at 349.815 kb on - strand, within acnA at 350.026 kb on + strand at 350.026 kb on + strand at 350.080 kb on - strand at 350.107 kb on + strand at 350.138 kb on - strand at 350.140 kb on - strand at 350.148 kb on - strand at 350.148 kb on - strand at 350.224 kb on - strand at 350.229 kb on - strand at 350.337 kb on + strand at 350.345 kb on - strand, within prpC at 350.364 kb on + strand, within prpC at 350.388 kb on + strand, within prpC at 350.398 kb on + strand, within prpC at 350.414 kb on + strand, within prpC at 350.551 kb on + strand, within prpC at 350.559 kb on - strand, within prpC at 350.559 kb on - strand, within prpC at 350.562 kb on + strand, within prpC at 350.615 kb on - strand, within prpC at 350.623 kb on + strand, within prpC at 350.695 kb on - strand, within prpC at 350.777 kb on + strand, within prpC at 350.792 kb on - strand, within prpC at 350.842 kb on + strand, within prpC at 350.842 kb on + strand, within prpC at 350.850 kb on - strand, within prpC at 351.029 kb on - strand, within prpC at 351.029 kb on - strand, within prpC at 351.029 kb on - strand, within prpC at 351.029 kb on - strand, within prpC at 351.045 kb on - strand, within prpC at 351.115 kb on + strand, within prpC
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.125 mg/ml remove 346,670 - SO0342 0.34 -0.8 346,670 - SO0342 0.34 -1.8 346,702 - SO0342 0.37 -2.2 346,710 + SO0342 0.37 -1.7 346,743 + SO0342 0.40 -1.9 346,743 + SO0342 0.40 -3.5 346,743 + SO0342 0.40 -1.7 346,751 - SO0342 0.41 -3.3 346,935 - SO0342 0.56 -2.7 347,051 + SO0342 0.66 -2.9 347,148 - SO0342 0.74 -2.5 347,184 - SO0342 0.77 -3.7 347,237 - SO0342 0.81 -2.0 347,285 + SO0342 0.86 -3.0 347,297 + SO0342 0.87 -3.5 347,354 + -0.9 347,362 - -1.1 347,362 - -4.1 347,428 + -1.2 347,649 - -3.2 347,696 - -3.3 347,740 - -2.6 347,795 + -3.4 347,876 - acnA SO0343 0.13 -3.2 347,889 + acnA SO0343 0.13 -1.5 347,897 - acnA SO0343 0.14 -3.5 347,901 + acnA SO0343 0.14 -3.4 347,907 + acnA SO0343 0.14 -0.8 347,909 - acnA SO0343 0.14 -3.2 347,951 + acnA SO0343 0.16 -2.3 347,980 + acnA SO0343 0.17 -2.2 348,102 - acnA SO0343 0.21 -3.2 348,231 + acnA SO0343 0.26 -0.9 348,343 + acnA SO0343 0.31 -3.1 348,407 + acnA SO0343 0.33 -1.5 348,412 + acnA SO0343 0.33 -1.3 348,412 + acnA SO0343 0.33 -2.3 348,415 - acnA SO0343 0.33 -1.6 348,420 - acnA SO0343 0.34 -2.4 348,420 - acnA SO0343 0.34 -2.7 348,420 - acnA SO0343 0.34 -3.1 348,420 - acnA SO0343 0.34 -2.8 348,557 + acnA SO0343 0.39 -3.0 348,600 - acnA SO0343 0.41 -2.7 348,600 - acnA SO0343 0.41 -1.5 348,600 - acnA SO0343 0.41 -2.2 348,707 - acnA SO0343 0.45 -0.4 348,873 + acnA SO0343 0.51 -2.2 348,891 + acnA SO0343 0.52 -2.2 348,944 - acnA SO0343 0.54 -3.2 348,965 - acnA SO0343 0.55 -1.2 349,015 - acnA SO0343 0.56 -3.3 349,015 - acnA SO0343 0.56 -2.7 349,048 + acnA SO0343 0.58 -3.7 349,048 + acnA SO0343 0.58 -2.9 349,063 + acnA SO0343 0.58 -1.6 349,071 - acnA SO0343 0.59 -1.9 349,087 + acnA SO0343 0.59 -1.5 349,122 + acnA SO0343 0.61 -2.9 349,164 - acnA SO0343 0.62 -2.9 349,164 - acnA SO0343 0.62 -3.4 349,172 - acnA SO0343 0.63 -3.9 349,195 - acnA SO0343 0.63 -3.5 349,224 - acnA SO0343 0.65 -2.0 349,224 - acnA SO0343 0.65 -3.4 349,224 - acnA SO0343 0.65 -1.3 349,291 - acnA SO0343 0.67 -3.5 349,420 - acnA SO0343 0.72 -2.0 349,460 + acnA SO0343 0.74 -2.3 349,460 + acnA SO0343 0.74 -1.5 349,534 - acnA SO0343 0.76 -2.7 349,540 + acnA SO0343 0.77 -4.0 349,815 - acnA SO0343 0.87 -4.1 350,026 + -1.7 350,026 + -3.8 350,080 - -3.0 350,107 + -2.3 350,138 - -1.3 350,140 - -1.8 350,148 - -1.7 350,148 - -3.7 350,224 - +0.7 350,229 - -0.1 350,337 + +0.0 350,345 - prpC SO0344 0.10 -1.1 350,364 + prpC SO0344 0.12 +0.3 350,388 + prpC SO0344 0.14 +1.3 350,398 + prpC SO0344 0.15 +0.0 350,414 + prpC SO0344 0.16 +0.0 350,551 + prpC SO0344 0.29 -1.0 350,559 - prpC SO0344 0.29 -0.1 350,559 - prpC SO0344 0.29 +0.2 350,562 + prpC SO0344 0.30 +0.2 350,615 - prpC SO0344 0.34 -0.1 350,623 + prpC SO0344 0.35 -0.1 350,695 - prpC SO0344 0.41 -0.2 350,777 + prpC SO0344 0.49 +0.4 350,792 - prpC SO0344 0.50 +0.6 350,842 + prpC SO0344 0.54 +0.5 350,842 + prpC SO0344 0.54 +0.4 350,850 - prpC SO0344 0.55 -0.1 351,029 - prpC SO0344 0.71 +0.4 351,029 - prpC SO0344 0.71 +0.0 351,029 - prpC SO0344 0.71 +0.5 351,029 - prpC SO0344 0.71 +0.3 351,045 - prpC SO0344 0.72 +0.1 351,115 + prpC SO0344 0.79 -1.0
Or see this region's nucleotide sequence