Experiment: Paraquat dichloride 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gspE and gspF are separated by 3 nucleotides gspF and gspG are separated by 74 nucleotides gspG and gspH are separated by 1 nucleotides
SO0167: gspE - general secretion pathway protein E (NCBI ptt file), at 174,412 to 175,977
gspE
SO0168: gspF - general secretion pathway protein F (NCBI ptt file), at 175,981 to 177,204
gspF
SO0169: gspG - general secretion pathway protein G (NCBI ptt file), at 177,279 to 177,713
gspG
SO0170: gspH - general secretion pathway protein H (NCBI ptt file), at 177,715 to 178,287
gspH
Position (kb) 175
176
177
178 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 174.999 kb on + strand, within gspE at 174.999 kb on + strand, within gspE at 174.999 kb on + strand, within gspE at 175.007 kb on - strand, within gspE at 175.013 kb on - strand, within gspE at 175.269 kb on + strand, within gspE at 175.285 kb on - strand, within gspE at 175.407 kb on - strand, within gspE at 175.451 kb on - strand, within gspE at 175.484 kb on - strand, within gspE at 175.484 kb on - strand, within gspE at 175.550 kb on - strand, within gspE at 175.570 kb on + strand, within gspE at 175.578 kb on + strand, within gspE at 175.710 kb on + strand, within gspE at 175.817 kb on + strand, within gspE at 175.951 kb on + strand at 175.959 kb on - strand at 175.972 kb on + strand at 175.979 kb on - strand at 175.980 kb on - strand at 175.980 kb on - strand at 176.005 kb on - strand at 176.010 kb on + strand at 176.025 kb on + strand at 176.033 kb on - strand at 176.033 kb on - strand at 176.134 kb on + strand, within gspF at 176.229 kb on - strand, within gspF at 176.247 kb on + strand, within gspF at 176.247 kb on + strand, within gspF at 176.255 kb on - strand, within gspF at 176.255 kb on - strand, within gspF at 176.255 kb on - strand, within gspF at 176.255 kb on - strand, within gspF at 176.255 kb on - strand, within gspF at 176.260 kb on + strand, within gspF at 176.289 kb on - strand, within gspF at 176.327 kb on + strand, within gspF at 176.327 kb on + strand, within gspF at 176.335 kb on - strand, within gspF at 176.382 kb on - strand, within gspF at 176.382 kb on - strand, within gspF at 176.524 kb on - strand, within gspF at 176.555 kb on - strand, within gspF at 176.597 kb on - strand, within gspF at 176.640 kb on - strand, within gspF at 176.795 kb on - strand, within gspF at 176.834 kb on + strand, within gspF at 176.834 kb on + strand, within gspF at 176.936 kb on + strand, within gspF at 176.941 kb on + strand, within gspF at 176.941 kb on + strand, within gspF at 176.941 kb on + strand, within gspF at 176.953 kb on + strand, within gspF at 176.958 kb on + strand, within gspF at 176.958 kb on + strand, within gspF at 176.966 kb on - strand, within gspF at 176.966 kb on - strand, within gspF at 177.010 kb on - strand, within gspF at 177.118 kb on + strand at 177.269 kb on + strand at 177.277 kb on - strand at 177.303 kb on + strand at 177.303 kb on + strand at 177.329 kb on + strand, within gspG at 177.364 kb on - strand, within gspG at 177.366 kb on - strand, within gspG at 177.397 kb on - strand, within gspG at 177.476 kb on + strand, within gspG at 177.492 kb on + strand, within gspG at 177.554 kb on + strand, within gspG at 177.613 kb on - strand, within gspG at 177.638 kb on + strand, within gspG at 177.662 kb on + strand, within gspG at 177.751 kb on - strand at 177.788 kb on + strand, within gspH at 177.837 kb on + strand, within gspH at 177.837 kb on + strand, within gspH at 177.837 kb on + strand, within gspH at 177.845 kb on - strand, within gspH at 177.845 kb on - strand, within gspH at 178.065 kb on - strand, within gspH
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.125 mg/ml remove 174,999 + gspE SO0167 0.37 -0.7 174,999 + gspE SO0167 0.37 -1.6 174,999 + gspE SO0167 0.37 -1.7 175,007 - gspE SO0167 0.38 -0.9 175,013 - gspE SO0167 0.38 -0.6 175,269 + gspE SO0167 0.55 -0.5 175,285 - gspE SO0167 0.56 -1.6 175,407 - gspE SO0167 0.64 -0.7 175,451 - gspE SO0167 0.66 -1.1 175,484 - gspE SO0167 0.68 -1.7 175,484 - gspE SO0167 0.68 -2.2 175,550 - gspE SO0167 0.73 +0.3 175,570 + gspE SO0167 0.74 -0.7 175,578 + gspE SO0167 0.74 -1.5 175,710 + gspE SO0167 0.83 -2.7 175,817 + gspE SO0167 0.90 -2.0 175,951 + -0.2 175,959 - -1.2 175,972 + +0.1 175,979 - -0.6 175,980 - -1.1 175,980 - -0.5 176,005 - -1.9 176,010 + -1.5 176,025 + -1.2 176,033 - -0.8 176,033 - -1.0 176,134 + gspF SO0168 0.12 -2.9 176,229 - gspF SO0168 0.20 -1.5 176,247 + gspF SO0168 0.22 -3.8 176,247 + gspF SO0168 0.22 -0.1 176,255 - gspF SO0168 0.22 +0.0 176,255 - gspF SO0168 0.22 -1.7 176,255 - gspF SO0168 0.22 -1.4 176,255 - gspF SO0168 0.22 -0.6 176,255 - gspF SO0168 0.22 +0.6 176,260 + gspF SO0168 0.23 -0.7 176,289 - gspF SO0168 0.25 -1.0 176,327 + gspF SO0168 0.28 -0.5 176,327 + gspF SO0168 0.28 -1.6 176,335 - gspF SO0168 0.29 -1.0 176,382 - gspF SO0168 0.33 -1.8 176,382 - gspF SO0168 0.33 +0.1 176,524 - gspF SO0168 0.44 -1.9 176,555 - gspF SO0168 0.47 -1.7 176,597 - gspF SO0168 0.50 -1.0 176,640 - gspF SO0168 0.54 -0.1 176,795 - gspF SO0168 0.67 -0.8 176,834 + gspF SO0168 0.70 -2.6 176,834 + gspF SO0168 0.70 -1.3 176,936 + gspF SO0168 0.78 -0.7 176,941 + gspF SO0168 0.78 +1.0 176,941 + gspF SO0168 0.78 -0.8 176,941 + gspF SO0168 0.78 -1.4 176,953 + gspF SO0168 0.79 -0.6 176,958 + gspF SO0168 0.80 -2.0 176,958 + gspF SO0168 0.80 -0.7 176,966 - gspF SO0168 0.80 -1.0 176,966 - gspF SO0168 0.80 -1.3 177,010 - gspF SO0168 0.84 -0.5 177,118 + -1.1 177,269 + +0.1 177,277 - -1.3 177,303 + -1.0 177,303 + -0.1 177,329 + gspG SO0169 0.11 -2.5 177,364 - gspG SO0169 0.20 -0.7 177,366 - gspG SO0169 0.20 -0.8 177,397 - gspG SO0169 0.27 -0.2 177,476 + gspG SO0169 0.45 -0.2 177,492 + gspG SO0169 0.49 -1.2 177,554 + gspG SO0169 0.63 +0.1 177,613 - gspG SO0169 0.77 -0.8 177,638 + gspG SO0169 0.83 -1.3 177,662 + gspG SO0169 0.88 -0.3 177,751 - -1.6 177,788 + gspH SO0170 0.13 -1.0 177,837 + gspH SO0170 0.21 -0.9 177,837 + gspH SO0170 0.21 +0.6 177,837 + gspH SO0170 0.21 -0.5 177,845 - gspH SO0170 0.23 -1.8 177,845 - gspH SO0170 0.23 -0.8 178,065 - gspH SO0170 0.61 -1.4
Or see this region's nucleotide sequence