Experiment: Cephalothin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1959 and Shew_1960 are separated by 38 nucleotides Shew_1960 and Shew_1961 are separated by 162 nucleotides Shew_1961 and Shew_1962 are separated by 107 nucleotides
Shew_1959: Shew_1959 - short chain dehydrogenase (RefSeq), at 2,264,022 to 2,264,837
_1959
Shew_1960: Shew_1960 - hypothetical protein (RefSeq), at 2,264,876 to 2,265,418
_1960
Shew_1961: Shew_1961 - acyl-CoA dehydrogenase domain-containing protein (RefSeq), at 2,265,581 to 2,267,332
_1961
Shew_1962: Shew_1962 - D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase (RefSeq), at 2,267,440 to 2,269,071
_1962
Position (kb) 2265
2266
2267
2268 Strain fitness (log2 ratio) -1
0
1
2
3 at 2264.915 kb on - strand at 2265.246 kb on - strand, within Shew_1960 at 2265.783 kb on + strand, within Shew_1961 at 2266.095 kb on + strand, within Shew_1961 at 2266.496 kb on - strand, within Shew_1961 at 2267.254 kb on + strand at 2267.262 kb on - strand at 2267.897 kb on - strand, within Shew_1962 at 2267.978 kb on + strand, within Shew_1962
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.05 mg/ml remove 2,264,915 - +1.4 2,265,246 - Shew_1960 0.68 -0.7 2,265,783 + Shew_1961 0.12 +3.6 2,266,095 + Shew_1961 0.29 +2.1 2,266,496 - Shew_1961 0.52 +0.1 2,267,254 + +0.1 2,267,262 - +0.4 2,267,897 - Shew_1962 0.28 -1.2 2,267,978 + Shew_1962 0.33 -0.3
Or see this region's nucleotide sequence