Experiment: Cephalothin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1628 and Shew_1629 are separated by 39 nucleotides Shew_1629 and Shew_1630 overlap by 8 nucleotides Shew_1630 and Shew_1631 are separated by 75 nucleotides Shew_1631 and Shew_1632 are separated by 95 nucleotides
Shew_1628: Shew_1628 - acylphosphatase (RefSeq), at 1,861,653 to 1,861,922
_1628
Shew_1629: Shew_1629 - hypothetical protein (RefSeq), at 1,861,962 to 1,862,900
_1629
Shew_1630: Shew_1630 - transcription-repair coupling factor (RefSeq), at 1,862,893 to 1,866,375
_1630
Shew_1631: Shew_1631 - hypothetical protein (RefSeq), at 1,866,451 to 1,867,032
_1631
Shew_1632: Shew_1632 - LolC/E family lipoprotein releasing system, transmembrane protein (RefSeq), at 1,867,128 to 1,868,360
_1632
Position (kb) 1862
1863
1864
1865
1866
1867 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1862.387 kb on - strand, within Shew_1629 at 1865.318 kb on - strand, within Shew_1630 at 1865.719 kb on + strand, within Shew_1630 at 1866.327 kb on + strand at 1866.571 kb on + strand, within Shew_1631 at 1866.625 kb on - strand, within Shew_1631 at 1866.649 kb on + strand, within Shew_1631 at 1866.973 kb on - strand, within Shew_1631
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.05 mg/ml remove 1,862,387 - Shew_1629 0.45 -2.1 1,865,318 - Shew_1630 0.70 -3.1 1,865,719 + Shew_1630 0.81 -2.5 1,866,327 + -1.8 1,866,571 + Shew_1631 0.21 +1.5 1,866,625 - Shew_1631 0.30 +0.9 1,866,649 + Shew_1631 0.34 +0.1 1,866,973 - Shew_1631 0.90 +0.5
Or see this region's nucleotide sequence