Experiment: Cephalothin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0855 and Shew_0856 are separated by 113 nucleotides Shew_0856 and Shew_0857 are separated by 134 nucleotides Shew_0857 and Shew_0858 are separated by 129 nucleotides Shew_0858 and Shew_0859 are separated by 10 nucleotides
Shew_0855: Shew_0855 - hypothetical protein (RefSeq), at 977,957 to 978,166
_0855
Shew_0856: Shew_0856 - phosphoglycerate mutase (RefSeq), at 978,280 to 978,816
_0856
Shew_0857: Shew_0857 - hypothetical protein (RefSeq), at 978,951 to 979,550
_0857
Shew_0858: Shew_0858 - oxidoreductase FAD/NAD(P)-binding subunit (RefSeq), at 979,680 to 980,420
_0858
Shew_0859: Shew_0859 - glutathione S-transferase domain-containing protein (RefSeq), at 980,431 to 981,030
_0859
Position (kb) 978
979
980 Strain fitness (log2 ratio) -1
0
1 at 978.259 kb on + strand at 978.434 kb on + strand, within Shew_0856 at 978.442 kb on - strand, within Shew_0856 at 978.473 kb on + strand, within Shew_0856 at 978.673 kb on + strand, within Shew_0856 at 978.678 kb on + strand, within Shew_0856 at 979.013 kb on + strand, within Shew_0857 at 979.282 kb on - strand, within Shew_0857 at 979.553 kb on - strand at 979.642 kb on - strand at 979.989 kb on - strand, within Shew_0858 at 979.991 kb on + strand, within Shew_0858 at 980.452 kb on + strand at 980.463 kb on + strand at 980.463 kb on + strand at 980.471 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.05 mg/ml remove 978,259 + +0.1 978,434 + Shew_0856 0.29 -0.4 978,442 - Shew_0856 0.30 +0.2 978,473 + Shew_0856 0.36 -0.8 978,673 + Shew_0856 0.73 -0.4 978,678 + Shew_0856 0.74 +0.3 979,013 + Shew_0857 0.10 -0.3 979,282 - Shew_0857 0.55 +0.2 979,553 - -0.5 979,642 - +0.0 979,989 - Shew_0858 0.42 -0.1 979,991 + Shew_0858 0.42 +0.6 980,452 + -0.0 980,463 + -0.6 980,463 + -0.4 980,471 - +0.1
Or see this region's nucleotide sequence