Experiment: rich media with hydrogen peroxide 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2759 and Dsui_2760 are separated by 349 nucleotides Dsui_2760 and Dsui_2761 are separated by 97 nucleotides Dsui_2761 and Dsui_2762 are separated by 162 nucleotides Dsui_2762 and Dsui_2763 are separated by 64 nucleotides
Dsui_2759: Dsui_2759 - pseudouridine synthase family protein, at 2,918,917 to 2,920,515
_2759
Dsui_2760: Dsui_2760 - translation initiation factor IF-1, at 2,920,865 to 2,921,125
_2760
Dsui_2761: Dsui_2761 - hypothetical protein, at 2,921,223 to 2,921,387
_2761
Dsui_2762: Dsui_2762 - Protein of unknown function (DUF3617), at 2,921,550 to 2,922,095
_2762
Dsui_2763: Dsui_2763 - ribosome biogenesis GTP-binding protein YlqF, at 2,922,160 to 2,923,074
_2763
Position (kb)
2921
2922 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2920.235 kb on - strand at 2920.250 kb on - strand at 2920.278 kb on + strand, within Dsui_2759 at 2920.286 kb on - strand at 2920.569 kb on + strand at 2920.606 kb on + strand at 2920.730 kb on + strand at 2921.200 kb on + strand at 2921.208 kb on - strand at 2921.369 kb on + strand, within Dsui_2761 at 2921.498 kb on + strand at 2921.560 kb on - strand at 2921.584 kb on - strand at 2921.598 kb on - strand at 2921.854 kb on + strand, within Dsui_2762 at 2921.896 kb on - strand at 2922.036 kb on - strand, within Dsui_2762 at 2922.097 kb on - strand at 2922.235 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction rich media with hydrogen peroxide 5 mM remove 2,920,235 - +0.2 2,920,250 - +0.8 2,920,278 + Dsui_2759 0.85 +0.3 2,920,286 - +2.8 2,920,569 + -0.3 2,920,606 + +0.7 2,920,730 + +0.5 2,921,200 + +1.6 2,921,208 - +0.6 2,921,369 + Dsui_2761 0.88 +0.4 2,921,498 + -1.0 2,921,560 - -0.1 2,921,584 - +1.4 2,921,598 - -0.6 2,921,854 + Dsui_2762 0.56 -0.2 2,921,896 - -0.5 2,922,036 - Dsui_2762 0.89 +0.6 2,922,097 - +0.0 2,922,235 + +0.1
Or see this region's nucleotide sequence