Experiment: rich media with hydrogen peroxide 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1953 and Dsui_1954 are separated by 3 nucleotides Dsui_1954 and Dsui_1955 are separated by 5 nucleotides Dsui_1955 and Dsui_1956 overlap by 4 nucleotides Dsui_1956 and Dsui_1957 are separated by 83 nucleotides Dsui_1957 and Dsui_1958 are separated by 152 nucleotides
Dsui_1953: Dsui_1953 - ADP-L-glycero-D-manno-heptose-6-epimerase, at 2,064,896 to 2,065,888
_1953
Dsui_1954: Dsui_1954 - cysteine synthase B, at 2,065,892 to 2,066,791
_1954
Dsui_1955: Dsui_1955 - 3'-5' exonuclease, at 2,066,797 to 2,067,594
_1955
Dsui_1956: Dsui_1956 - Rhodanese-related sulfurtransferase, at 2,067,591 to 2,068,034
_1956
Dsui_1957: Dsui_1957 - tRNA-Val, at 2,068,118 to 2,068,194
_1957
Dsui_1958: Dsui_1958 - threonyl-tRNA synthetase, at 2,068,347 to 2,070,263
_1958
Position (kb)
2066
2067
2068 Strain fitness (log2 ratio)
-2
-1
0
1 at 2066.794 kb on - strand at 2066.831 kb on + strand at 2066.906 kb on + strand, within Dsui_1955 at 2066.906 kb on + strand, within Dsui_1955 at 2066.976 kb on + strand, within Dsui_1955 at 2066.978 kb on + strand, within Dsui_1955 at 2067.176 kb on + strand, within Dsui_1955 at 2067.176 kb on + strand, within Dsui_1955 at 2067.327 kb on + strand, within Dsui_1955 at 2067.351 kb on + strand, within Dsui_1955 at 2067.432 kb on + strand, within Dsui_1955 at 2067.437 kb on + strand, within Dsui_1955 at 2067.444 kb on + strand, within Dsui_1955 at 2067.476 kb on - strand, within Dsui_1955 at 2067.643 kb on + strand, within Dsui_1956 at 2067.651 kb on - strand at 2067.651 kb on - strand, within Dsui_1956 at 2067.682 kb on + strand, within Dsui_1956 at 2067.715 kb on + strand, within Dsui_1956 at 2067.723 kb on + strand, within Dsui_1956 at 2067.768 kb on - strand, within Dsui_1956 at 2067.811 kb on + strand, within Dsui_1956 at 2067.821 kb on + strand, within Dsui_1956 at 2067.821 kb on + strand, within Dsui_1956 at 2067.829 kb on - strand, within Dsui_1956 at 2067.829 kb on - strand, within Dsui_1956 at 2067.929 kb on + strand, within Dsui_1956 at 2067.937 kb on - strand, within Dsui_1956 at 2068.012 kb on - strand at 2068.066 kb on + strand at 2068.074 kb on - strand at 2068.074 kb on - strand at 2068.074 kb on - strand at 2068.207 kb on + strand at 2068.209 kb on + strand at 2068.253 kb on + strand at 2068.325 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction rich media with hydrogen peroxide 5 mM remove 2,066,794 - -0.3 2,066,831 + +0.5 2,066,906 + Dsui_1955 0.14 -1.7 2,066,906 + Dsui_1955 0.14 -2.1 2,066,976 + Dsui_1955 0.22 -1.4 2,066,978 + Dsui_1955 0.23 -2.3 2,067,176 + Dsui_1955 0.47 -1.3 2,067,176 + Dsui_1955 0.47 -0.6 2,067,327 + Dsui_1955 0.66 +0.5 2,067,351 + Dsui_1955 0.69 -1.4 2,067,432 + Dsui_1955 0.80 +0.7 2,067,437 + Dsui_1955 0.80 -0.9 2,067,444 + Dsui_1955 0.81 -0.5 2,067,476 - Dsui_1955 0.85 -0.5 2,067,643 + Dsui_1956 0.12 +0.1 2,067,651 - +0.7 2,067,651 - Dsui_1956 0.14 -0.1 2,067,682 + Dsui_1956 0.20 +0.5 2,067,715 + Dsui_1956 0.28 -0.4 2,067,723 + Dsui_1956 0.30 -0.1 2,067,768 - Dsui_1956 0.40 +1.4 2,067,811 + Dsui_1956 0.50 +0.6 2,067,821 + Dsui_1956 0.52 +0.2 2,067,821 + Dsui_1956 0.52 -0.0 2,067,829 - Dsui_1956 0.54 +0.6 2,067,829 - Dsui_1956 0.54 -0.5 2,067,929 + Dsui_1956 0.76 +0.1 2,067,937 - Dsui_1956 0.78 +0.6 2,068,012 - -0.1 2,068,066 + -0.0 2,068,074 - +0.2 2,068,074 - -0.5 2,068,074 - +0.5 2,068,207 + -0.0 2,068,209 + -0.5 2,068,253 + -0.1 2,068,325 + -0.6
Or see this region's nucleotide sequence