Experiment: rich media with hydrogen peroxide 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0468 and Dsui_0469 overlap by 4 nucleotides Dsui_0469 and Dsui_0470 are separated by 31 nucleotides Dsui_0470 and Dsui_0471 overlap by 16 nucleotides
Dsui_0468: Dsui_0468 - glycosyltransferase, at 475,326 to 476,465
_0468
Dsui_0469: Dsui_0469 - lipopolysaccharide heptosyltransferase III, putative, at 476,462 to 477,565
_0469
Dsui_0470: Dsui_0470 - fructose-2,6-bisphosphatase, at 477,597 to 478,166
_0470
Dsui_0471: Dsui_0471 - cobalamin 5''-phosphate synthase/cobalamin synthase, at 478,151 to 478,927
_0471
Position (kb) 476
477
478 Strain fitness (log2 ratio) -1
0
1 at 475.590 kb on - strand, within Dsui_0468 at 477.003 kb on + strand at 477.052 kb on - strand, within Dsui_0469 at 477.113 kb on - strand, within Dsui_0469 at 477.165 kb on - strand, within Dsui_0469 at 477.463 kb on - strand at 477.688 kb on + strand, within Dsui_0470 at 477.696 kb on - strand, within Dsui_0470 at 477.696 kb on - strand, within Dsui_0470 at 477.798 kb on + strand at 477.918 kb on - strand, within Dsui_0470 at 477.995 kb on + strand, within Dsui_0470 at 478.134 kb on + strand at 478.169 kb on - strand at 478.169 kb on - strand at 478.199 kb on + strand at 478.325 kb on - strand, within Dsui_0471 at 478.341 kb on + strand at 478.442 kb on - strand, within Dsui_0471
Per-strain Table
Position Strand Gene LocusTag Fraction rich media with hydrogen peroxide 5 mM remove 475,590 - Dsui_0468 0.23 -0.4 477,003 + -1.0 477,052 - Dsui_0469 0.53 -0.7 477,113 - Dsui_0469 0.59 -0.7 477,165 - Dsui_0469 0.64 +0.1 477,463 - -0.4 477,688 + Dsui_0470 0.16 -0.2 477,696 - Dsui_0470 0.17 +0.4 477,696 - Dsui_0470 0.17 +0.6 477,798 + +0.6 477,918 - Dsui_0470 0.56 +1.2 477,995 + Dsui_0470 0.70 +0.6 478,134 + +1.0 478,169 - -1.0 478,169 - +0.4 478,199 + -1.0 478,325 - Dsui_0471 0.22 -0.2 478,341 + +0.0 478,442 - Dsui_0471 0.37 +1.2
Or see this region's nucleotide sequence