Experiment: A22 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_R0074 and Shew_R0075 are separated by 42 nucleotides Shew_R0075 and Shew_R0076 are separated by 26 nucleotides Shew_R0076 and Shew_R0077 are separated by 24 nucleotides Shew_R0077 and Shew_2065 are separated by 116 nucleotides Shew_2065 and Shew_2066 are separated by 83 nucleotides Shew_2066 and Shew_2067 are separated by 2 nucleotides
Shew_R0074: Shew_R0074 - tRNA-Asn (RefSeq), at 2,385,603 to 2,385,678
_R0074
Shew_R0075: Shew_R0075 - tRNA-Asn (RefSeq), at 2,385,721 to 2,385,796
_R0075
Shew_R0076: Shew_R0076 - tRNA-Asn (RefSeq), at 2,385,823 to 2,385,898
_R0076
Shew_R0077: Shew_R0077 - tRNA-Asn (RefSeq), at 2,385,923 to 2,385,998
_R0077
Shew_2065: Shew_2065 - diguanylate cyclase/phosphodiesterase (RefSeq), at 2,386,115 to 2,388,043
_2065
Shew_2066: Shew_2066 - Na(+)-translocating NADH-quinone reductase subunit E (RefSeq), at 2,388,127 to 2,388,705
_2066
Shew_2067: Shew_2067 - electron transport complex, RnfABCDGE type, B subunit (RefSeq), at 2,388,708 to 2,389,277
_2067
Position (kb)
2386
2387
2388
2389 Strain fitness (log2 ratio)
-1
0
1 at 2385.307 kb on + strand at 2385.371 kb on + strand at 2385.393 kb on + strand at 2385.881 kb on + strand, within Shew_R0076 at 2386.613 kb on + strand, within Shew_2065 at 2386.752 kb on - strand, within Shew_2065 at 2386.759 kb on + strand, within Shew_2065 at 2387.466 kb on + strand, within Shew_2065 at 2387.971 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.005 mg/ml remove 2,385,307 + +0.2 2,385,371 + -0.3 2,385,393 + +0.4 2,385,881 + Shew_R0076 0.76 -0.2 2,386,613 + Shew_2065 0.26 +0.6 2,386,752 - Shew_2065 0.33 +0.1 2,386,759 + Shew_2065 0.33 +0.4 2,387,466 + Shew_2065 0.70 +0.4 2,387,971 - +1.1
Or see this region's nucleotide sequence