Experiment: A22 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1543 and Shew_1544 are separated by 47 nucleotides Shew_1544 and Shew_1545 are separated by 153 nucleotides
Shew_1543: Shew_1543 - secretion protein HlyD family protein (RefSeq), at 1,771,815 to 1,772,873
_1543
Shew_1544: Shew_1544 - TetR family transcriptional regulator (RefSeq), at 1,772,921 to 1,773,580
_1544
Shew_1545: Shew_1545 - two component LuxR family transcriptional regulator (RefSeq), at 1,773,734 to 1,774,393
_1545
Position (kb)
1772
1773
1774 Strain fitness (log2 ratio)
-2
-1
0
1 at 1772.000 kb on - strand, within Shew_1543 at 1772.251 kb on + strand, within Shew_1543 at 1772.251 kb on + strand, within Shew_1543 at 1772.259 kb on - strand, within Shew_1543 at 1772.259 kb on - strand, within Shew_1543 at 1772.439 kb on + strand, within Shew_1543 at 1772.573 kb on - strand, within Shew_1543 at 1772.588 kb on - strand, within Shew_1543 at 1772.628 kb on + strand, within Shew_1543 at 1772.735 kb on - strand, within Shew_1543 at 1773.049 kb on + strand, within Shew_1544 at 1773.066 kb on + strand, within Shew_1544 at 1773.096 kb on + strand, within Shew_1544 at 1773.686 kb on + strand at 1773.740 kb on - strand at 1774.197 kb on - strand, within Shew_1545 at 1774.574 kb on + strand at 1774.574 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.005 mg/ml remove 1,772,000 - Shew_1543 0.17 -0.2 1,772,251 + Shew_1543 0.41 +1.3 1,772,251 + Shew_1543 0.41 +0.6 1,772,259 - Shew_1543 0.42 +0.1 1,772,259 - Shew_1543 0.42 +0.1 1,772,439 + Shew_1543 0.59 -0.2 1,772,573 - Shew_1543 0.72 +1.6 1,772,588 - Shew_1543 0.73 -0.7 1,772,628 + Shew_1543 0.77 -0.1 1,772,735 - Shew_1543 0.87 -0.4 1,773,049 + Shew_1544 0.19 -0.8 1,773,066 + Shew_1544 0.22 -2.2 1,773,096 + Shew_1544 0.27 -0.3 1,773,686 + +0.1 1,773,740 - -0.2 1,774,197 - Shew_1545 0.70 -0.3 1,774,574 + +0.2 1,774,574 + +0.2
Or see this region's nucleotide sequence