Experiment: A22 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1214 and recA are separated by 330 nucleotides recA and Shew_1216 are separated by 79 nucleotides Shew_1216 and Shew_1217 are separated by 244 nucleotides
Shew_1214: Shew_1214 - DNA mismatch repair protein (RefSeq), at 1,408,043 to 1,410,610
_1214
Shew_1215: recA - recombinase A (RefSeq), at 1,410,941 to 1,412,002
recA
Shew_1216: Shew_1216 - regulatory protein RecX (RefSeq), at 1,412,082 to 1,412,534
_1216
Shew_1217: Shew_1217 - alanyl-tRNA synthetase (RefSeq), at 1,412,779 to 1,415,403
_1217
Position (kb)
1410
1411
1412
1413 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1410.071 kb on - strand, within Shew_1214 at 1410.236 kb on + strand, within Shew_1214 at 1410.342 kb on + strand, within Shew_1214 at 1410.620 kb on + strand at 1410.783 kb on - strand at 1410.856 kb on + strand at 1410.856 kb on + strand at 1410.856 kb on + strand at 1410.856 kb on + strand at 1410.864 kb on - strand at 1410.864 kb on - strand at 1410.900 kb on + strand at 1411.185 kb on + strand, within recA at 1411.495 kb on + strand, within recA at 1411.549 kb on - strand, within recA at 1411.575 kb on + strand, within recA at 1412.153 kb on + strand, within Shew_1216 at 1412.291 kb on + strand, within Shew_1216 at 1412.294 kb on - strand, within Shew_1216 at 1412.304 kb on - strand, within Shew_1216 at 1412.455 kb on - strand, within Shew_1216
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.005 mg/ml remove 1,410,071 - Shew_1214 0.79 +0.8 1,410,236 + Shew_1214 0.85 +0.7 1,410,342 + Shew_1214 0.90 +0.4 1,410,620 + +0.9 1,410,783 - +0.1 1,410,856 + -0.2 1,410,856 + +0.8 1,410,856 + -4.7 1,410,856 + -0.8 1,410,864 - -3.2 1,410,864 - -2.2 1,410,900 + +0.8 1,411,185 + recA Shew_1215 0.23 -1.2 1,411,495 + recA Shew_1215 0.52 -2.6 1,411,549 - recA Shew_1215 0.57 +0.8 1,411,575 + recA Shew_1215 0.60 -0.8 1,412,153 + Shew_1216 0.16 +0.6 1,412,291 + Shew_1216 0.46 +0.1 1,412,294 - Shew_1216 0.47 +0.8 1,412,304 - Shew_1216 0.49 +2.7 1,412,455 - Shew_1216 0.82 -0.3
Or see this region's nucleotide sequence