Experiment: A22 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1209 and Shew_1210 overlap by 8 nucleotides Shew_1210 and Shew_1211 overlap by 19 nucleotides Shew_1211 and Shew_1212 are separated by 49 nucleotides
Shew_1209: Shew_1209 - pseudouridylate synthase (RefSeq), at 1,403,308 to 1,404,375
_1209
Shew_1210: Shew_1210 - stationary-phase survival protein SurE (RefSeq), at 1,404,368 to 1,405,117
_1210
Shew_1211: Shew_1211 - protein-L-isoaspartate O-methyltransferase (RefSeq), at 1,405,099 to 1,405,749
_1211
Shew_1212: Shew_1212 - peptidase M23B (RefSeq), at 1,405,799 to 1,406,731
_1212
Position (kb) 1405
1406 Strain fitness (log2 ratio) -1
0
1 at 1404.427 kb on + strand at 1404.481 kb on + strand, within Shew_1210 at 1404.569 kb on - strand, within Shew_1210 at 1405.267 kb on + strand, within Shew_1211 at 1405.302 kb on + strand, within Shew_1211 at 1405.310 kb on - strand, within Shew_1211 at 1405.544 kb on + strand, within Shew_1211 at 1405.995 kb on + strand, within Shew_1212 at 1406.079 kb on - strand, within Shew_1212 at 1406.562 kb on + strand, within Shew_1212
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.005 mg/ml remove 1,404,427 + +1.3 1,404,481 + Shew_1210 0.15 +0.9 1,404,569 - Shew_1210 0.27 +1.3 1,405,267 + Shew_1211 0.26 +0.5 1,405,302 + Shew_1211 0.31 +1.1 1,405,310 - Shew_1211 0.32 +1.1 1,405,544 + Shew_1211 0.68 +0.9 1,405,995 + Shew_1212 0.21 +0.2 1,406,079 - Shew_1212 0.30 +0.8 1,406,562 + Shew_1212 0.82 -0.5
Or see this region's nucleotide sequence