Experiment: A22 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1158 and Shew_1159 are separated by 271 nucleotides Shew_1159 and Shew_1160 are separated by 184 nucleotides Shew_1160 and Shew_1161 are separated by 158 nucleotides
Shew_1158: Shew_1158 - alkaline phosphatase (RefSeq), at 1,331,587 to 1,333,095
_1158
Shew_1159: Shew_1159 - thioesterase superfamily protein (RefSeq), at 1,333,367 to 1,333,735
_1159
Shew_1160: Shew_1160 - hypothetical protein (RefSeq), at 1,333,920 to 1,334,126
_1160
Shew_1161: Shew_1161 - NADH dehydrogenase (RefSeq), at 1,334,285 to 1,335,586
_1161
Position (kb)
1333
1334 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1332.387 kb on - strand, within Shew_1158 at 1332.472 kb on + strand, within Shew_1158 at 1332.605 kb on - strand, within Shew_1158 at 1332.764 kb on + strand, within Shew_1158 at 1332.827 kb on + strand, within Shew_1158 at 1332.879 kb on + strand, within Shew_1158 at 1332.887 kb on - strand, within Shew_1158 at 1332.887 kb on - strand, within Shew_1158 at 1332.887 kb on - strand, within Shew_1158 at 1333.017 kb on - strand at 1333.582 kb on + strand, within Shew_1159 at 1333.585 kb on - strand, within Shew_1159 at 1333.622 kb on - strand, within Shew_1159 at 1333.727 kb on - strand at 1333.793 kb on - strand at 1333.796 kb on + strand at 1333.806 kb on + strand at 1333.814 kb on - strand at 1334.283 kb on + strand at 1334.653 kb on - strand, within Shew_1161
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.005 mg/ml remove 1,332,387 - Shew_1158 0.53 +0.4 1,332,472 + Shew_1158 0.59 -1.8 1,332,605 - Shew_1158 0.67 +0.4 1,332,764 + Shew_1158 0.78 +0.2 1,332,827 + Shew_1158 0.82 -0.2 1,332,879 + Shew_1158 0.86 +0.0 1,332,887 - Shew_1158 0.86 -0.4 1,332,887 - Shew_1158 0.86 -0.9 1,332,887 - Shew_1158 0.86 -0.1 1,333,017 - +0.3 1,333,582 + Shew_1159 0.58 +0.2 1,333,585 - Shew_1159 0.59 -1.2 1,333,622 - Shew_1159 0.69 -1.1 1,333,727 - +0.9 1,333,793 - +3.9 1,333,796 + -1.2 1,333,806 + +0.0 1,333,814 - -0.1 1,334,283 + -0.2 1,334,653 - Shew_1161 0.28 +0.0
Or see this region's nucleotide sequence