Experiment: A22 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0729 and Shew_0730 are separated by 88 nucleotides Shew_0730 and Shew_0731 are separated by 8 nucleotides Shew_0731 and Shew_0732 are separated by 282 nucleotides
Shew_0729: Shew_0729 - FAD-dependent pyridine nucleotide-disulphide oxidoreductase (RefSeq), at 830,300 to 832,000
_0729
Shew_0730: Shew_0730 - protein of unknown function DUF395, YeeE/YedE (RefSeq), at 832,089 to 832,517
_0730
Shew_0731: Shew_0731 - protein of unknown function DUF395, YeeE/YedE (RefSeq), at 832,526 to 832,996
_0731
Shew_0732: Shew_0732 - diguanylate cyclase with PAS/PAC sensor (RefSeq), at 833,279 to 834,619
_0732
Position (kb) 832
833 Strain fitness (log2 ratio) -1
0
1
2 at 831.494 kb on - strand, within Shew_0729 at 831.644 kb on + strand, within Shew_0729 at 831.652 kb on - strand, within Shew_0729 at 832.113 kb on - strand at 832.123 kb on - strand at 832.467 kb on + strand, within Shew_0730 at 832.636 kb on + strand, within Shew_0731 at 832.724 kb on - strand, within Shew_0731 at 832.825 kb on - strand, within Shew_0731 at 833.142 kb on + strand at 833.469 kb on - strand, within Shew_0732 at 833.485 kb on + strand, within Shew_0732 at 833.485 kb on + strand, within Shew_0732
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.005 mg/ml remove 831,494 - Shew_0729 0.70 +0.3 831,644 + Shew_0729 0.79 +0.3 831,652 - Shew_0729 0.79 +2.6 832,113 - -0.7 832,123 - -0.2 832,467 + Shew_0730 0.88 -0.9 832,636 + Shew_0731 0.23 +0.3 832,724 - Shew_0731 0.42 +0.1 832,825 - Shew_0731 0.63 -0.9 833,142 + +2.2 833,469 - Shew_0732 0.14 +1.5 833,485 + Shew_0732 0.15 +0.3 833,485 + Shew_0732 0.15 -0.1
Or see this region's nucleotide sequence