Experiment: A22 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0315 and Shew_0316 overlap by 4 nucleotides Shew_0316 and Shew_0317 are separated by 55 nucleotides Shew_0317 and hemC are separated by 2 nucleotides
Shew_0315: Shew_0315 - HemY domain-containing protein (RefSeq), at 360,568 to 361,734
_0315
Shew_0316: Shew_0316 - protein of unknown function DUF513, hemX (RefSeq), at 361,731 to 362,873
_0316
Shew_0317: Shew_0317 - uroporphyrinogen III synthase HEM4 (RefSeq), at 362,929 to 363,660
_0317
Shew_0318: hemC - porphobilinogen deaminase (RefSeq), at 363,663 to 364,616
hemC
Position (kb) 361
362
363 Strain fitness (log2 ratio) -2
-1
0
1 at 361.178 kb on + strand, within Shew_0315 at 361.453 kb on + strand, within Shew_0315 at 361.770 kb on + strand at 361.962 kb on - strand, within Shew_0316 at 362.185 kb on - strand, within Shew_0316 at 362.185 kb on - strand, within Shew_0316 at 362.234 kb on - strand, within Shew_0316 at 362.452 kb on + strand, within Shew_0316 at 362.558 kb on + strand, within Shew_0316 at 362.884 kb on + strand at 362.949 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.005 mg/ml remove 361,178 + Shew_0315 0.52 -0.7 361,453 + Shew_0315 0.76 -0.2 361,770 + +0.0 361,962 - Shew_0316 0.20 -1.0 362,185 - Shew_0316 0.40 -2.4 362,185 - Shew_0316 0.40 -1.4 362,234 - Shew_0316 0.44 +0.2 362,452 + Shew_0316 0.63 -0.8 362,558 + Shew_0316 0.72 -0.7 362,884 + -0.5 362,949 - +0.4
Or see this region's nucleotide sequence