Experiment: m.b. copper (II) chloride 0.64 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_4084 and Dshi_4085 overlap by 14 nucleotides Dshi_4085 and Dshi_4086 are separated by 143 nucleotides Dshi_4086 and Dshi_4087 overlap by 14 nucleotides Dshi_4087 and Dshi_4088 are separated by 5 nucleotides Dshi_4088 and Dshi_4089 are separated by 161 nucleotides
Dshi_4084: Dshi_4084 - Resolvase domain (RefSeq), at 19,415 to 19,981
_4084
Dshi_4085: Dshi_4085 - WGR domain protein (RefSeq), at 19,968 to 20,222
_4085
Dshi_4086: Dshi_4086 - hypothetical protein (RefSeq), at 20,366 to 20,572
_4086
Dshi_4087: Dshi_4087 - plasmid stabilization system (RefSeq), at 20,559 to 20,852
_4087
Dshi_4088: Dshi_4088 - hypothetical protein (RefSeq), at 20,858 to 21,250
_4088
Dshi_4089: Dshi_4089 - Integrase catalytic region (RefSeq), at 21,412 to 22,221
_4089
Position (kb) 20
21 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 19.419 kb on + strand at 19.420 kb on + strand at 19.420 kb on + strand at 19.426 kb on - strand at 19.427 kb on - strand at 19.428 kb on - strand at 19.453 kb on + strand at 19.453 kb on + strand at 19.461 kb on - strand at 19.461 kb on - strand at 19.461 kb on - strand at 19.466 kb on - strand at 19.476 kb on - strand, within Dshi_4084 at 19.476 kb on - strand, within Dshi_4084 at 19.491 kb on + strand, within Dshi_4084 at 19.593 kb on + strand, within Dshi_4084 at 19.620 kb on - strand, within Dshi_4084 at 19.728 kb on + strand, within Dshi_4084 at 19.751 kb on - strand, within Dshi_4084 at 19.751 kb on - strand, within Dshi_4084 at 19.790 kb on + strand, within Dshi_4084 at 19.790 kb on + strand, within Dshi_4084 at 19.790 kb on + strand, within Dshi_4084 at 19.796 kb on - strand, within Dshi_4084 at 19.796 kb on - strand, within Dshi_4084 at 19.798 kb on - strand, within Dshi_4084 at 19.798 kb on - strand, within Dshi_4084 at 19.833 kb on + strand, within Dshi_4084 at 19.841 kb on - strand, within Dshi_4084 at 19.875 kb on + strand, within Dshi_4084 at 19.885 kb on + strand, within Dshi_4084 at 19.894 kb on - strand, within Dshi_4084 at 19.900 kb on + strand, within Dshi_4084 at 19.900 kb on + strand, within Dshi_4084 at 19.908 kb on - strand, within Dshi_4084 at 19.908 kb on - strand, within Dshi_4084 at 19.961 kb on - strand at 20.012 kb on + strand, within Dshi_4085 at 20.012 kb on + strand, within Dshi_4085 at 20.069 kb on + strand, within Dshi_4085 at 20.069 kb on + strand, within Dshi_4085 at 20.077 kb on - strand, within Dshi_4085 at 20.080 kb on + strand, within Dshi_4085 at 20.121 kb on - strand, within Dshi_4085 at 20.121 kb on - strand, within Dshi_4085 at 20.136 kb on - strand, within Dshi_4085 at 20.145 kb on + strand, within Dshi_4085 at 20.153 kb on - strand, within Dshi_4085 at 20.160 kb on + strand, within Dshi_4085 at 20.180 kb on + strand, within Dshi_4085 at 20.193 kb on - strand, within Dshi_4085 at 20.203 kb on - strand at 20.213 kb on + strand at 20.218 kb on + strand at 20.223 kb on + strand at 20.223 kb on + strand at 20.223 kb on + strand at 20.223 kb on + strand at 20.223 kb on + strand at 20.223 kb on + strand at 20.223 kb on + strand at 20.223 kb on + strand at 20.223 kb on + strand at 20.223 kb on + strand at 20.228 kb on + strand at 20.228 kb on + strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.231 kb on - strand at 20.233 kb on + strand at 20.236 kb on - strand at 20.236 kb on - strand at 20.236 kb on - strand at 20.241 kb on - strand at 20.253 kb on - strand at 20.300 kb on - strand at 20.302 kb on + strand at 20.551 kb on + strand, within Dshi_4086 at 20.566 kb on + strand at 20.566 kb on + strand at 20.566 kb on + strand at 20.574 kb on - strand at 20.585 kb on + strand at 20.593 kb on - strand, within Dshi_4087 at 20.600 kb on + strand, within Dshi_4087 at 20.640 kb on + strand, within Dshi_4087 at 20.640 kb on + strand, within Dshi_4087 at 20.640 kb on + strand, within Dshi_4087 at 20.677 kb on + strand, within Dshi_4087 at 20.677 kb on + strand, within Dshi_4087 at 20.677 kb on + strand, within Dshi_4087 at 20.678 kb on + strand, within Dshi_4087 at 20.685 kb on + strand, within Dshi_4087 at 20.685 kb on + strand, within Dshi_4087 at 20.685 kb on + strand, within Dshi_4087 at 20.685 kb on + strand, within Dshi_4087 at 20.685 kb on - strand, within Dshi_4087 at 20.685 kb on - strand, within Dshi_4087 at 20.696 kb on - strand, within Dshi_4087 at 20.698 kb on + strand, within Dshi_4087 at 20.698 kb on + strand, within Dshi_4087 at 20.699 kb on - strand, within Dshi_4087 at 20.699 kb on - strand, within Dshi_4087 at 20.700 kb on + strand, within Dshi_4087 at 20.700 kb on + strand, within Dshi_4087 at 20.700 kb on + strand, within Dshi_4087 at 20.700 kb on + strand, within Dshi_4087 at 20.700 kb on + strand, within Dshi_4087 at 20.700 kb on + strand, within Dshi_4087 at 20.706 kb on - strand, within Dshi_4087 at 20.716 kb on - strand, within Dshi_4087 at 20.718 kb on - strand, within Dshi_4087 at 20.718 kb on - strand, within Dshi_4087 at 20.718 kb on - strand, within Dshi_4087 at 20.736 kb on + strand, within Dshi_4087 at 20.736 kb on + strand, within Dshi_4087 at 20.736 kb on + strand, within Dshi_4087 at 20.747 kb on - strand, within Dshi_4087 at 20.762 kb on + strand, within Dshi_4087 at 20.771 kb on + strand, within Dshi_4087 at 20.793 kb on + strand, within Dshi_4087 at 20.801 kb on + strand, within Dshi_4087 at 20.816 kb on - strand, within Dshi_4087 at 20.833 kb on - strand at 20.870 kb on - strand at 20.871 kb on + strand at 20.905 kb on - strand, within Dshi_4088 at 20.945 kb on - strand, within Dshi_4088 at 20.946 kb on + strand, within Dshi_4088 at 20.949 kb on - strand, within Dshi_4088 at 20.951 kb on + strand, within Dshi_4088 at 20.951 kb on + strand, within Dshi_4088 at 20.951 kb on + strand, within Dshi_4088 at 20.951 kb on + strand, within Dshi_4088 at 20.951 kb on + strand, within Dshi_4088 at 20.951 kb on + strand, within Dshi_4088 at 20.952 kb on + strand, within Dshi_4088 at 20.961 kb on + strand, within Dshi_4088 at 21.000 kb on - strand, within Dshi_4088 at 21.017 kb on + strand, within Dshi_4088 at 21.017 kb on + strand, within Dshi_4088 at 21.024 kb on - strand, within Dshi_4088 at 21.026 kb on + strand, within Dshi_4088 at 21.026 kb on + strand, within Dshi_4088 at 21.047 kb on + strand, within Dshi_4088 at 21.057 kb on + strand, within Dshi_4088 at 21.059 kb on - strand, within Dshi_4088 at 21.065 kb on - strand, within Dshi_4088 at 21.129 kb on - strand, within Dshi_4088 at 21.150 kb on + strand, within Dshi_4088 at 21.152 kb on + strand, within Dshi_4088 at 21.165 kb on + strand, within Dshi_4088 at 21.165 kb on - strand, within Dshi_4088 at 21.173 kb on - strand, within Dshi_4088 at 21.173 kb on - strand, within Dshi_4088 at 21.173 kb on - strand, within Dshi_4088 at 21.173 kb on - strand, within Dshi_4088 at 21.173 kb on - strand, within Dshi_4088 at 21.173 kb on - strand, within Dshi_4088 at 21.173 kb on - strand, within Dshi_4088 at 21.203 kb on - strand, within Dshi_4088 at 21.228 kb on + strand at 21.234 kb on + strand at 21.240 kb on + strand at 21.243 kb on - strand at 21.255 kb on + strand at 21.255 kb on + strand at 21.263 kb on - strand at 21.330 kb on + strand at 21.344 kb on + strand at 21.349 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. copper (II) chloride 0.64 mM remove 19,419 + -1.6 19,420 + +0.6 19,420 + +2.0 19,426 - +2.2 19,427 - +0.0 19,428 - -0.4 19,453 + -0.4 19,453 + +0.9 19,461 - +0.5 19,461 - +0.9 19,461 - -0.1 19,466 - -0.8 19,476 - Dshi_4084 0.11 -1.5 19,476 - Dshi_4084 0.11 +1.3 19,491 + Dshi_4084 0.13 +0.4 19,593 + Dshi_4084 0.31 -0.6 19,620 - Dshi_4084 0.36 -1.1 19,728 + Dshi_4084 0.55 -2.0 19,751 - Dshi_4084 0.59 +0.6 19,751 - Dshi_4084 0.59 -3.5 19,790 + Dshi_4084 0.66 +1.0 19,790 + Dshi_4084 0.66 -0.2 19,790 + Dshi_4084 0.66 +0.0 19,796 - Dshi_4084 0.67 +0.8 19,796 - Dshi_4084 0.67 +0.9 19,798 - Dshi_4084 0.68 -0.6 19,798 - Dshi_4084 0.68 +1.0 19,833 + Dshi_4084 0.74 -0.9 19,841 - Dshi_4084 0.75 +0.0 19,875 + Dshi_4084 0.81 +0.4 19,885 + Dshi_4084 0.83 +0.5 19,894 - Dshi_4084 0.84 -0.3 19,900 + Dshi_4084 0.86 -0.6 19,900 + Dshi_4084 0.86 +1.0 19,908 - Dshi_4084 0.87 +0.5 19,908 - Dshi_4084 0.87 +4.9 19,961 - +0.1 20,012 + Dshi_4085 0.17 -0.2 20,012 + Dshi_4085 0.17 +0.0 20,069 + Dshi_4085 0.40 -0.3 20,069 + Dshi_4085 0.40 -4.2 20,077 - Dshi_4085 0.43 +1.4 20,080 + Dshi_4085 0.44 +2.6 20,121 - Dshi_4085 0.60 +0.4 20,121 - Dshi_4085 0.60 +0.8 20,136 - Dshi_4085 0.66 -0.4 20,145 + Dshi_4085 0.69 +0.3 20,153 - Dshi_4085 0.73 -0.6 20,160 + Dshi_4085 0.75 +1.3 20,180 + Dshi_4085 0.83 +4.5 20,193 - Dshi_4085 0.88 -0.2 20,203 - -0.7 20,213 + -1.2 20,218 + -2.4 20,223 + -1.0 20,223 + +0.0 20,223 + -0.4 20,223 + -0.2 20,223 + +0.6 20,223 + -1.0 20,223 + +0.1 20,223 + +1.0 20,223 + -0.3 20,223 + +1.0 20,228 + +2.5 20,228 + -1.1 20,231 - +1.3 20,231 - +0.2 20,231 - +3.1 20,231 - +1.0 20,231 - -2.6 20,231 - -0.7 20,231 - -2.3 20,231 - +0.6 20,231 - +1.6 20,231 - -0.6 20,231 - +1.0 20,231 - +0.1 20,231 - +0.3 20,233 + +0.9 20,236 - +0.9 20,236 - +0.0 20,236 - -0.1 20,241 - +0.0 20,253 - -1.6 20,300 - +0.0 20,302 + -0.1 20,551 + Dshi_4086 0.89 +1.1 20,566 + -1.3 20,566 + -0.7 20,566 + +3.8 20,574 - +0.7 20,585 + +1.3 20,593 - Dshi_4087 0.12 +0.1 20,600 + Dshi_4087 0.14 -0.7 20,640 + Dshi_4087 0.28 -0.9 20,640 + Dshi_4087 0.28 -0.4 20,640 + Dshi_4087 0.28 -0.5 20,677 + Dshi_4087 0.40 -1.8 20,677 + Dshi_4087 0.40 -0.2 20,677 + Dshi_4087 0.40 +0.0 20,678 + Dshi_4087 0.40 +0.8 20,685 + Dshi_4087 0.43 +3.3 20,685 + Dshi_4087 0.43 +0.3 20,685 + Dshi_4087 0.43 +0.7 20,685 + Dshi_4087 0.43 -0.2 20,685 - Dshi_4087 0.43 -1.2 20,685 - Dshi_4087 0.43 +0.0 20,696 - Dshi_4087 0.47 +2.0 20,698 + Dshi_4087 0.47 +0.3 20,698 + Dshi_4087 0.47 +2.2 20,699 - Dshi_4087 0.48 -0.5 20,699 - Dshi_4087 0.48 -2.6 20,700 + Dshi_4087 0.48 +1.0 20,700 + Dshi_4087 0.48 +1.4 20,700 + Dshi_4087 0.48 -0.4 20,700 + Dshi_4087 0.48 -0.6 20,700 + Dshi_4087 0.48 -1.5 20,700 + Dshi_4087 0.48 +0.6 20,706 - Dshi_4087 0.50 +0.6 20,716 - Dshi_4087 0.53 +1.4 20,718 - Dshi_4087 0.54 -1.5 20,718 - Dshi_4087 0.54 -0.8 20,718 - Dshi_4087 0.54 -0.3 20,736 + Dshi_4087 0.60 +0.6 20,736 + Dshi_4087 0.60 +0.4 20,736 + Dshi_4087 0.60 +0.2 20,747 - Dshi_4087 0.64 -2.0 20,762 + Dshi_4087 0.69 -1.3 20,771 + Dshi_4087 0.72 +0.4 20,793 + Dshi_4087 0.80 -0.3 20,801 + Dshi_4087 0.82 +0.5 20,816 - Dshi_4087 0.87 +0.1 20,833 - +1.0 20,870 - -0.4 20,871 + -1.9 20,905 - Dshi_4088 0.12 +1.0 20,945 - Dshi_4088 0.22 +1.1 20,946 + Dshi_4088 0.22 -1.8 20,949 - Dshi_4088 0.23 +0.8 20,951 + Dshi_4088 0.24 +0.0 20,951 + Dshi_4088 0.24 -0.5 20,951 + Dshi_4088 0.24 -0.4 20,951 + Dshi_4088 0.24 -1.9 20,951 + Dshi_4088 0.24 -1.6 20,951 + Dshi_4088 0.24 -0.1 20,952 + Dshi_4088 0.24 -1.2 20,961 + Dshi_4088 0.26 -1.3 21,000 - Dshi_4088 0.36 -1.0 21,017 + Dshi_4088 0.40 +0.4 21,017 + Dshi_4088 0.40 +1.6 21,024 - Dshi_4088 0.42 +0.5 21,026 + Dshi_4088 0.43 +0.4 21,026 + Dshi_4088 0.43 +3.2 21,047 + Dshi_4088 0.48 -0.3 21,057 + Dshi_4088 0.51 -0.5 21,059 - Dshi_4088 0.51 +0.1 21,065 - Dshi_4088 0.53 -1.2 21,129 - Dshi_4088 0.69 +0.8 21,150 + Dshi_4088 0.74 -0.1 21,152 + Dshi_4088 0.75 -2.2 21,165 + Dshi_4088 0.78 -0.3 21,165 - Dshi_4088 0.78 -0.6 21,173 - Dshi_4088 0.80 +1.6 21,173 - Dshi_4088 0.80 -0.5 21,173 - Dshi_4088 0.80 -0.4 21,173 - Dshi_4088 0.80 +4.5 21,173 - Dshi_4088 0.80 +1.4 21,173 - Dshi_4088 0.80 -2.8 21,173 - Dshi_4088 0.80 -0.9 21,203 - Dshi_4088 0.88 +0.3 21,228 + -1.4 21,234 + -0.5 21,240 + +1.0 21,243 - -1.4 21,255 + +0.6 21,255 + +0.1 21,263 - -0.2 21,330 + -0.2 21,344 + -0.3 21,349 + -0.5
Or see this region's nucleotide sequence