Experiment: m.b. copper (II) chloride 0.64 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3815 and Dshi_3816 are separated by 2 nucleotides Dshi_3816 and Dshi_3817 are separated by 125 nucleotides Dshi_3817 and Dshi_3818 are separated by 17 nucleotides Dshi_3818 and Dshi_3819 overlap by 4 nucleotides
Dshi_3815: Dshi_3815 - monooxygenase FAD-binding (RefSeq), at 23,077 to 24,702
_3815
Dshi_3816: Dshi_3816 - hypothetical protein (RefSeq), at 24,705 to 24,890
_3816
Dshi_3817: Dshi_3817 - beta-ketoadipyl CoA thiolase (RefSeq), at 25,016 to 26,218
_3817
Dshi_3818: Dshi_3818 - phenylacetate-CoA oxygenase, PaaG subunit (RefSeq), at 26,236 to 27,213
_3818
Dshi_3819: Dshi_3819 - phenylacetate-CoA oxygenase, PaaH subunit (RefSeq), at 27,210 to 27,542
_3819
Position (kb)
25
26
27 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 24.135 kb on - strand, within Dshi_3815 at 24.137 kb on - strand, within Dshi_3815 at 24.139 kb on + strand, within Dshi_3815 at 24.157 kb on - strand, within Dshi_3815 at 24.165 kb on + strand, within Dshi_3815 at 24.165 kb on + strand, within Dshi_3815 at 24.173 kb on - strand, within Dshi_3815 at 24.259 kb on + strand, within Dshi_3815 at 24.267 kb on - strand, within Dshi_3815 at 24.439 kb on - strand, within Dshi_3815 at 24.632 kb on - strand at 24.721 kb on - strand at 24.721 kb on - strand at 25.144 kb on + strand, within Dshi_3817 at 25.153 kb on - strand, within Dshi_3817 at 25.313 kb on - strand, within Dshi_3817 at 25.409 kb on + strand, within Dshi_3817 at 25.410 kb on + strand, within Dshi_3817 at 25.430 kb on + strand, within Dshi_3817 at 25.695 kb on - strand, within Dshi_3817 at 25.928 kb on + strand, within Dshi_3817 at 25.978 kb on + strand, within Dshi_3817 at 26.002 kb on - strand, within Dshi_3817 at 26.019 kb on + strand, within Dshi_3817 at 26.111 kb on + strand at 26.144 kb on + strand at 26.196 kb on - strand at 26.212 kb on + strand at 26.325 kb on - strand at 26.365 kb on + strand, within Dshi_3818 at 26.532 kb on - strand, within Dshi_3818 at 26.561 kb on + strand, within Dshi_3818 at 26.611 kb on + strand, within Dshi_3818 at 26.619 kb on - strand, within Dshi_3818 at 26.649 kb on + strand, within Dshi_3818 at 26.722 kb on + strand, within Dshi_3818 at 26.770 kb on - strand, within Dshi_3818 at 26.823 kb on - strand, within Dshi_3818 at 26.874 kb on - strand, within Dshi_3818 at 26.880 kb on - strand, within Dshi_3818 at 26.956 kb on + strand, within Dshi_3818 at 26.963 kb on + strand, within Dshi_3818 at 26.997 kb on + strand, within Dshi_3818
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. copper (II) chloride 0.64 mM remove 24,135 - Dshi_3815 0.65 -2.2 24,137 - Dshi_3815 0.65 -2.2 24,139 + Dshi_3815 0.65 -1.6 24,157 - Dshi_3815 0.66 +1.8 24,165 + Dshi_3815 0.67 -0.2 24,165 + Dshi_3815 0.67 -0.2 24,173 - Dshi_3815 0.67 +1.8 24,259 + Dshi_3815 0.73 +2.8 24,267 - Dshi_3815 0.73 -0.8 24,439 - Dshi_3815 0.84 -0.3 24,632 - +0.2 24,721 - -1.8 24,721 - +1.8 25,144 + Dshi_3817 0.11 -1.8 25,153 - Dshi_3817 0.11 -2.0 25,313 - Dshi_3817 0.25 -0.2 25,409 + Dshi_3817 0.33 -3.9 25,410 + Dshi_3817 0.33 -1.8 25,430 + Dshi_3817 0.34 -0.2 25,695 - Dshi_3817 0.56 -0.6 25,928 + Dshi_3817 0.76 +0.2 25,978 + Dshi_3817 0.80 +0.8 26,002 - Dshi_3817 0.82 -2.2 26,019 + Dshi_3817 0.83 -2.7 26,111 + -0.5 26,144 + +0.7 26,196 - -2.6 26,212 + +0.4 26,325 - -0.2 26,365 + Dshi_3818 0.13 +2.2 26,532 - Dshi_3818 0.30 -1.2 26,561 + Dshi_3818 0.33 +0.7 26,611 + Dshi_3818 0.38 -2.5 26,619 - Dshi_3818 0.39 -0.7 26,649 + Dshi_3818 0.42 -0.8 26,722 + Dshi_3818 0.50 -1.5 26,770 - Dshi_3818 0.55 -0.2 26,823 - Dshi_3818 0.60 +0.2 26,874 - Dshi_3818 0.65 +0.6 26,880 - Dshi_3818 0.66 -0.8 26,956 + Dshi_3818 0.74 -1.4 26,963 + Dshi_3818 0.74 +1.2 26,997 + Dshi_3818 0.78 +0.1
Or see this region's nucleotide sequence