Experiment: m.b. copper (II) chloride 0.64 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0185 and Dshi_0186 overlap by 4 nucleotides Dshi_0186 and Dshi_0187 are separated by 22 nucleotides Dshi_0187 and Dshi_0188 are separated by 662 nucleotides
Dshi_0185: Dshi_0185 - phosphatidate cytidylyltransferase (RefSeq), at 186,744 to 187,694
_0185
Dshi_0186: Dshi_0186 - phospholipid/glycerol acyltransferase (RefSeq), at 187,691 to 188,344
_0186
Dshi_0187: Dshi_0187 - Lysine exporter protein (LYSE/YGGA) (RefSeq), at 188,367 to 188,981
_0187
Dshi_0188: Dshi_0188 - hydroxymethylbutenyl pyrophosphate reductase (RefSeq), at 189,644 to 190,600
_0188
Position (kb)
188
189 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 187.543 kb on + strand, within Dshi_0185 at 187.774 kb on + strand at 187.806 kb on + strand, within Dshi_0186 at 187.814 kb on - strand, within Dshi_0186 at 188.180 kb on + strand, within Dshi_0186 at 188.411 kb on - strand at 188.618 kb on + strand, within Dshi_0187 at 188.818 kb on - strand, within Dshi_0187 at 188.911 kb on + strand, within Dshi_0187 at 188.918 kb on - strand, within Dshi_0187 at 189.014 kb on - strand at 189.044 kb on + strand at 189.052 kb on - strand at 189.075 kb on - strand at 189.145 kb on + strand at 189.152 kb on + strand at 189.160 kb on - strand at 189.169 kb on - strand at 189.172 kb on - strand at 189.172 kb on - strand at 189.172 kb on - strand at 189.179 kb on + strand at 189.218 kb on - strand at 189.413 kb on + strand at 189.554 kb on - strand at 189.570 kb on + strand at 189.578 kb on - strand at 189.578 kb on - strand at 189.588 kb on - strand at 189.618 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. copper (II) chloride 0.64 mM remove 187,543 + Dshi_0185 0.84 -1.0 187,774 + -1.3 187,806 + Dshi_0186 0.18 -2.0 187,814 - Dshi_0186 0.19 -1.7 188,180 + Dshi_0186 0.75 -1.7 188,411 - -3.1 188,618 + Dshi_0187 0.41 +0.3 188,818 - Dshi_0187 0.73 +0.3 188,911 + Dshi_0187 0.88 +3.5 188,918 - Dshi_0187 0.90 -0.3 189,014 - -2.4 189,044 + +0.5 189,052 - +0.3 189,075 - +0.3 189,145 + -0.3 189,152 + +0.7 189,160 - +1.3 189,169 - +0.3 189,172 - +2.5 189,172 - -0.7 189,172 - +2.3 189,179 + +3.3 189,218 - +1.9 189,413 + +2.3 189,554 - -0.5 189,570 + -0.7 189,578 - -0.7 189,578 - -1.4 189,588 - +0.3 189,618 - -1.7
Or see this region's nucleotide sequence