Experiment: m.b. copper (II) chloride 0.64 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0179 and Dshi_0180 are separated by 72 nucleotides Dshi_0180 and Dshi_0181 overlap by 4 nucleotides Dshi_0181 and Dshi_0182 are separated by 104 nucleotides Dshi_0182 and Dshi_0183 overlap by 8 nucleotides
Dshi_0179: Dshi_0179 - peptide deformylase (RefSeq), at 183,047 to 183,610
_0179
Dshi_0180: Dshi_0180 - peptide deformylase (RefSeq), at 183,683 to 184,189
_0180
Dshi_0181: Dshi_0181 - methionyl-tRNA formyltransferase (RefSeq), at 184,186 to 185,085
_0181
Dshi_0182: Dshi_0182 - ribonuclease H (RefSeq), at 185,190 to 185,663
_0182
Dshi_0183: Dshi_0183 - Methyltransferase type 11 (RefSeq), at 185,656 to 186,255
_0183
Position (kb) 184
185
186 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 183.760 kb on + strand, within Dshi_0180 at 183.827 kb on + strand, within Dshi_0180 at 183.959 kb on - strand, within Dshi_0180 at 184.057 kb on + strand, within Dshi_0180 at 184.077 kb on - strand, within Dshi_0180 at 184.090 kb on + strand, within Dshi_0180 at 184.168 kb on + strand at 184.193 kb on - strand at 185.057 kb on + strand at 185.109 kb on + strand at 185.189 kb on - strand at 185.597 kb on - strand, within Dshi_0182 at 185.678 kb on - strand at 185.801 kb on + strand, within Dshi_0183 at 185.801 kb on + strand, within Dshi_0183 at 185.844 kb on + strand, within Dshi_0183
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. copper (II) chloride 0.64 mM remove 183,760 + Dshi_0180 0.15 -1.7 183,827 + Dshi_0180 0.28 -2.7 183,959 - Dshi_0180 0.54 -0.7 184,057 + Dshi_0180 0.74 -1.3 184,077 - Dshi_0180 0.78 +2.9 184,090 + Dshi_0180 0.80 -1.7 184,168 + +1.3 184,193 - -2.0 185,057 + +1.5 185,109 + +0.3 185,189 - +0.3 185,597 - Dshi_0182 0.86 +2.3 185,678 - -1.8 185,801 + Dshi_0183 0.24 -0.8 185,801 + Dshi_0183 0.24 +0.3 185,844 + Dshi_0183 0.31 +0.6
Or see this region's nucleotide sequence