Experiment: m.b. copper (II) chloride 0.64 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0178 and Dshi_0179 are separated by 73 nucleotides Dshi_0179 and Dshi_0180 are separated by 72 nucleotides Dshi_0180 and Dshi_0181 overlap by 4 nucleotides
Dshi_0178: Dshi_0178 - aminotransferase class I and II (RefSeq), at 181,801 to 182,973
_0178
Dshi_0179: Dshi_0179 - peptide deformylase (RefSeq), at 183,047 to 183,610
_0179
Dshi_0180: Dshi_0180 - peptide deformylase (RefSeq), at 183,683 to 184,189
_0180
Dshi_0181: Dshi_0181 - methionyl-tRNA formyltransferase (RefSeq), at 184,186 to 185,085
_0181
Position (kb) 183
184 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 182.351 kb on - strand, within Dshi_0178 at 182.361 kb on - strand, within Dshi_0178 at 182.438 kb on + strand, within Dshi_0178 at 182.438 kb on + strand, within Dshi_0178 at 182.530 kb on + strand, within Dshi_0178 at 182.539 kb on - strand at 182.539 kb on - strand, within Dshi_0178 at 182.563 kb on + strand, within Dshi_0178 at 182.609 kb on + strand, within Dshi_0178 at 182.668 kb on + strand, within Dshi_0178 at 182.730 kb on - strand, within Dshi_0178 at 182.770 kb on + strand, within Dshi_0178 at 182.874 kb on + strand at 182.939 kb on + strand at 182.947 kb on - strand at 182.968 kb on + strand at 182.976 kb on - strand at 182.988 kb on - strand at 183.760 kb on + strand, within Dshi_0180 at 183.827 kb on + strand, within Dshi_0180 at 183.959 kb on - strand, within Dshi_0180 at 184.057 kb on + strand, within Dshi_0180 at 184.077 kb on - strand, within Dshi_0180 at 184.090 kb on + strand, within Dshi_0180 at 184.168 kb on + strand at 184.193 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. copper (II) chloride 0.64 mM remove 182,351 - Dshi_0178 0.47 -1.7 182,361 - Dshi_0178 0.48 -0.8 182,438 + Dshi_0178 0.54 -0.8 182,438 + Dshi_0178 0.54 +0.6 182,530 + Dshi_0178 0.62 -1.3 182,539 - -0.7 182,539 - Dshi_0178 0.63 -4.0 182,563 + Dshi_0178 0.65 -0.7 182,609 + Dshi_0178 0.69 -2.0 182,668 + Dshi_0178 0.74 +0.2 182,730 - Dshi_0178 0.79 +0.7 182,770 + Dshi_0178 0.83 +0.8 182,874 + +1.3 182,939 + -2.0 182,947 - -1.7 182,968 + -2.3 182,976 - +0.4 182,988 - +0.9 183,760 + Dshi_0180 0.15 -1.7 183,827 + Dshi_0180 0.28 -2.7 183,959 - Dshi_0180 0.54 -0.7 184,057 + Dshi_0180 0.74 -1.3 184,077 - Dshi_0180 0.78 +2.9 184,090 + Dshi_0180 0.80 -1.7 184,168 + +1.3 184,193 - -2.0
Or see this region's nucleotide sequence