Strain Fitness in Dinoroseobacter shibae DFL-12 around Dshi_0027

Experiment: m.b. copper (II) chloride 0.64 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntDshi_0025 and Dshi_0026 are separated by 73 nucleotidesDshi_0026 and Dshi_0027 are separated by 123 nucleotidesDshi_0027 and Dshi_0028 are separated by 7 nucleotides Dshi_0025: Dshi_0025 - anti-ECFsigma factor, ChrR (RefSeq), at 29,143 to 29,814 _0025 Dshi_0026: Dshi_0026 - NAD-dependent epimerase/dehydratase (RefSeq), at 29,888 to 30,529 _0026 Dshi_0027: Dshi_0027 - Alkane 1-monooxygenase (RefSeq), at 30,653 to 31,807 _0027 Dshi_0028: Dshi_0028 - A/G-specific adenine glycosylase (RefSeq), at 31,815 to 32,909 _0028 Position (kb) 30 31 32Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 29.667 kb on + strand, within Dshi_0025at 29.723 kb on + strand, within Dshi_0025at 29.731 kb on - strandat 29.821 kb on - strandat 29.822 kb on - strandat 29.838 kb on - strandat 29.996 kb on + strand, within Dshi_0026at 30.039 kb on - strand, within Dshi_0026at 30.258 kb on - strand, within Dshi_0026at 30.314 kb on + strand, within Dshi_0026at 30.337 kb on - strand, within Dshi_0026at 30.341 kb on + strand, within Dshi_0026at 30.341 kb on + strand, within Dshi_0026at 30.351 kb on + strand, within Dshi_0026at 30.395 kb on + strand, within Dshi_0026at 30.403 kb on - strand, within Dshi_0026at 30.419 kb on + strand, within Dshi_0026at 30.703 kb on - strandat 30.756 kb on + strandat 30.780 kb on + strand, within Dshi_0027at 30.811 kb on + strand, within Dshi_0027at 30.821 kb on + strand, within Dshi_0027at 30.836 kb on + strand, within Dshi_0027at 30.837 kb on + strand, within Dshi_0027at 30.857 kb on - strand, within Dshi_0027at 30.920 kb on + strand, within Dshi_0027at 30.982 kb on - strand, within Dshi_0027at 30.982 kb on - strand, within Dshi_0027at 31.019 kb on - strand, within Dshi_0027at 31.102 kb on - strand, within Dshi_0027at 31.145 kb on + strand, within Dshi_0027at 31.192 kb on + strand, within Dshi_0027at 31.197 kb on + strand, within Dshi_0027at 31.234 kb on + strand, within Dshi_0027at 31.308 kb on + strand, within Dshi_0027at 31.335 kb on + strand, within Dshi_0027at 31.343 kb on - strand, within Dshi_0027at 31.350 kb on + strand, within Dshi_0027at 31.377 kb on - strand, within Dshi_0027at 31.611 kb on + strand, within Dshi_0027at 31.803 kb on + strandat 31.847 kb on - strandat 31.868 kb on - strandat 31.868 kb on - strandat 31.959 kb on + strand, within Dshi_0028at 32.097 kb on + strand, within Dshi_0028at 32.166 kb on - strand, within Dshi_0028at 32.166 kb on - strand, within Dshi_0028at 32.249 kb on + strand, within Dshi_0028at 32.376 kb on - strand, within Dshi_0028at 32.520 kb on - strand, within Dshi_0028at 32.629 kb on + strand, within Dshi_0028at 32.678 kb on + strand, within Dshi_0028

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Per-strain Table

Position Strand Gene LocusTag Fraction m.b. copper (II) chloride 0.64 mM
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29,667 + Dshi_0025 0.78 -1.3
29,723 + Dshi_0025 0.86 -2.5
29,731 - -0.7
29,821 - +1.5
29,822 - -1.3
29,838 - +0.9
29,996 + Dshi_0026 0.17 -1.5
30,039 - Dshi_0026 0.24 +1.9
30,258 - Dshi_0026 0.58 -1.7
30,314 + Dshi_0026 0.66 +0.3
30,337 - Dshi_0026 0.70 +0.7
30,341 + Dshi_0026 0.71 -0.2
30,341 + Dshi_0026 0.71 -2.5
30,351 + Dshi_0026 0.72 -1.3
30,395 + Dshi_0026 0.79 -1.5
30,403 - Dshi_0026 0.80 -2.0
30,419 + Dshi_0026 0.83 -3.9
30,703 - +0.3
30,756 + +0.3
30,780 + Dshi_0027 0.11 -0.9
30,811 + Dshi_0027 0.14 -2.0
30,821 + Dshi_0027 0.15 +1.4
30,836 + Dshi_0027 0.16 -2.3
30,837 + Dshi_0027 0.16 -2.3
30,857 - Dshi_0027 0.18 -2.1
30,920 + Dshi_0027 0.23 +2.3
30,982 - Dshi_0027 0.28 -0.1
30,982 - Dshi_0027 0.28 -2.8
31,019 - Dshi_0027 0.32 +0.3
31,102 - Dshi_0027 0.39 -1.0
31,145 + Dshi_0027 0.43 -0.7
31,192 + Dshi_0027 0.47 +1.9
31,197 + Dshi_0027 0.47 -3.3
31,234 + Dshi_0027 0.50 +0.3
31,308 + Dshi_0027 0.57 +1.7
31,335 + Dshi_0027 0.59 -0.5
31,343 - Dshi_0027 0.60 +0.3
31,350 + Dshi_0027 0.60 -1.3
31,377 - Dshi_0027 0.63 -2.0
31,611 + Dshi_0027 0.83 -1.0
31,803 + +1.3
31,847 - -1.3
31,868 - -2.3
31,868 - -3.7
31,959 + Dshi_0028 0.13 +1.9
32,097 + Dshi_0028 0.26 -2.8
32,166 - Dshi_0028 0.32 -1.0
32,166 - Dshi_0028 0.32 +0.9
32,249 + Dshi_0028 0.40 +0.7
32,376 - Dshi_0028 0.51 -0.7
32,520 - Dshi_0028 0.64 +1.9
32,629 + Dshi_0028 0.74 -0.1
32,678 + Dshi_0028 0.79 -0.9

Or see this region's nucleotide sequence