Experiment: sodium fluoride 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt umuC and umuD are separated by 0 nucleotides umuD and SOA0014 are separated by 592 nucleotides SOA0014 and SOA0015 are separated by 304 nucleotides SOA0015 and SOA0016 are separated by 66 nucleotides
SOA0012: umuC - umuC protein (NCBI ptt file), at 10,078 to 11,334
umuC
SOA0013: umuD - umuD protein (VIMSS), at 11,335 to 11,739
umuD
SOA0014: SOA0014 - hypothetical protein (NCBI ptt file), at 12,332 to 12,427
SOA0014
SOA0015: SOA0015 - hypothetical protein (NCBI ptt file), at 12,732 to 12,878
SOA0015
SOA0016: SOA0016 - ISSod9, transposase (NCBI ptt file), at 12,945 to 15,911
SOA0016
Position (kb)
12
13 Strain fitness (log2 ratio)
-2
-1
0
1 at 11.332 kb on + strand at 11.391 kb on - strand, within umuD at 11.452 kb on - strand, within umuD at 11.471 kb on - strand, within umuD at 11.585 kb on - strand, within umuD at 11.654 kb on + strand, within umuD at 11.660 kb on + strand, within umuD at 11.668 kb on - strand, within umuD at 11.678 kb on - strand, within umuD at 11.682 kb on - strand, within umuD at 11.913 kb on + strand at 12.111 kb on + strand at 12.111 kb on + strand at 12.113 kb on + strand at 12.119 kb on - strand at 12.120 kb on - strand at 12.120 kb on - strand at 12.204 kb on - strand at 12.293 kb on + strand at 12.301 kb on - strand at 12.301 kb on - strand at 12.303 kb on + strand at 12.330 kb on - strand at 12.359 kb on - strand, within SOA0014 at 12.361 kb on + strand, within SOA0014 at 12.561 kb on + strand at 12.569 kb on - strand at 12.592 kb on + strand at 12.686 kb on + strand at 12.686 kb on + strand at 12.904 kb on + strand at 12.912 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction sodium fluoride 50 mM remove 11,332 + -1.1 11,391 - umuD SOA0013 0.14 -0.4 11,452 - umuD SOA0013 0.29 -1.0 11,471 - umuD SOA0013 0.34 -0.7 11,585 - umuD SOA0013 0.62 -0.1 11,654 + umuD SOA0013 0.79 -0.5 11,660 + umuD SOA0013 0.80 -0.9 11,668 - umuD SOA0013 0.82 -0.7 11,678 - umuD SOA0013 0.85 -0.7 11,682 - umuD SOA0013 0.86 -0.6 11,913 + +1.0 12,111 + -0.2 12,111 + -0.6 12,113 + -0.9 12,119 - -0.7 12,120 - -0.3 12,120 - +1.5 12,204 - -0.6 12,293 + -0.2 12,301 - -0.4 12,301 - -0.7 12,303 + -0.7 12,330 - -1.8 12,359 - SOA0014 0.28 -1.1 12,361 + SOA0014 0.30 -1.0 12,561 + -0.6 12,569 - -0.2 12,592 + -0.4 12,686 + +0.2 12,686 + -1.3 12,904 + -0.5 12,912 - +0.1
Or see this region's nucleotide sequence