Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3423 and Shewana3_3424 are separated by 234 nucleotides Shewana3_3424 and Shewana3_3425 are separated by 1 nucleotides Shewana3_3425 and Shewana3_3426 overlap by 10 nucleotides Shewana3_3426 and Shewana3_3427 are separated by 2 nucleotides Shewana3_3427 and Shewana3_3428 are separated by 15 nucleotides
Shewana3_3423: Shewana3_3423 - hypothetical protein (RefSeq), at 4,100,456 to 4,101,706
_3423
Shewana3_3424: Shewana3_3424 - methylation site containing protein (RefSeq), at 4,101,941 to 4,102,312
_3424
Shewana3_3425: Shewana3_3425 - methylation site containing protein (RefSeq), at 4,102,314 to 4,102,748
_3425
Shewana3_3426: Shewana3_3426 - prepilin-type cleavage/methylation-like protein (RefSeq), at 4,102,739 to 4,103,146
_3426
Shewana3_3427: Shewana3_3427 - prepilin-type cleavage/methylation-like protein (RefSeq), at 4,103,149 to 4,103,691
_3427
Shewana3_3428: Shewana3_3428 - hypothetical protein (RefSeq), at 4,103,707 to 4,105,332
_3428
Position (kb)
4102
4103 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4101.419 kb on + strand, within Shewana3_3423 at 4101.734 kb on - strand at 4101.907 kb on + strand at 4101.960 kb on + strand at 4101.968 kb on - strand at 4102.137 kb on - strand, within Shewana3_3424 at 4102.137 kb on - strand, within Shewana3_3424 at 4102.335 kb on + strand at 4102.345 kb on - strand at 4102.941 kb on + strand, within Shewana3_3426 at 4103.023 kb on - strand, within Shewana3_3426 at 4103.259 kb on + strand, within Shewana3_3427 at 4103.297 kb on - strand, within Shewana3_3427 at 4103.313 kb on + strand, within Shewana3_3427 at 4103.491 kb on - strand, within Shewana3_3427 at 4103.521 kb on - strand, within Shewana3_3427 at 4103.651 kb on - strand at 4103.728 kb on - strand at 4103.733 kb on - strand at 4103.733 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 4,101,419 + Shewana3_3423 0.77 +0.6 4,101,734 - +0.3 4,101,907 + +0.2 4,101,960 + +0.0 4,101,968 - +0.1 4,102,137 - Shewana3_3424 0.53 -1.8 4,102,137 - Shewana3_3424 0.53 -4.3 4,102,335 + -1.0 4,102,345 - -1.4 4,102,941 + Shewana3_3426 0.50 -1.2 4,103,023 - Shewana3_3426 0.70 +0.1 4,103,259 + Shewana3_3427 0.20 -1.1 4,103,297 - Shewana3_3427 0.27 +0.0 4,103,313 + Shewana3_3427 0.30 +0.7 4,103,491 - Shewana3_3427 0.63 -1.6 4,103,521 - Shewana3_3427 0.69 +0.1 4,103,651 - +0.6 4,103,728 - -0.3 4,103,733 - +0.5 4,103,733 - -0.2
Or see this region's nucleotide sequence