Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3106 and Shewana3_3107 are separated by 50 nucleotides Shewana3_3107 and Shewana3_3108 are separated by 23 nucleotides Shewana3_3108 and Shewana3_3109 are separated by 185 nucleotides
Shewana3_3106: Shewana3_3106 - beta alanine--pyruvate transaminase (RefSeq), at 3,725,351 to 3,726,691
_3106
Shewana3_3107: Shewana3_3107 - methylmalonate-semialdehyde dehydrogenase [acylating] (RefSeq), at 3,726,742 to 3,728,232
_3107
Shewana3_3108: Shewana3_3108 - protein of unknown function DUF861, cupin_3 (RefSeq), at 3,728,256 to 3,728,615
_3108
Shewana3_3109: Shewana3_3109 - hypothetical protein (RefSeq), at 3,728,801 to 3,729,523
_3109
Position (kb) 3726
3727
3728
3729 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 3725.787 kb on + strand, within Shewana3_3106 at 3725.797 kb on + strand, within Shewana3_3106 at 3725.883 kb on + strand, within Shewana3_3106 at 3725.912 kb on - strand, within Shewana3_3106 at 3725.969 kb on + strand, within Shewana3_3106 at 3725.970 kb on + strand, within Shewana3_3106 at 3725.990 kb on + strand, within Shewana3_3106 at 3725.990 kb on + strand, within Shewana3_3106 at 3726.291 kb on + strand, within Shewana3_3106 at 3726.318 kb on + strand, within Shewana3_3106 at 3726.413 kb on - strand, within Shewana3_3106 at 3726.413 kb on - strand, within Shewana3_3106 at 3726.561 kb on + strand at 3726.561 kb on + strand at 3726.659 kb on + strand at 3726.996 kb on + strand, within Shewana3_3107 at 3727.047 kb on - strand, within Shewana3_3107 at 3727.252 kb on - strand, within Shewana3_3107 at 3727.311 kb on + strand, within Shewana3_3107 at 3727.364 kb on + strand, within Shewana3_3107 at 3727.437 kb on + strand, within Shewana3_3107 at 3727.555 kb on + strand, within Shewana3_3107 at 3727.563 kb on - strand, within Shewana3_3107 at 3727.573 kb on - strand, within Shewana3_3107 at 3727.851 kb on + strand, within Shewana3_3107 at 3727.900 kb on - strand, within Shewana3_3107 at 3727.929 kb on - strand, within Shewana3_3107 at 3727.931 kb on + strand, within Shewana3_3107 at 3728.018 kb on + strand, within Shewana3_3107 at 3728.023 kb on + strand, within Shewana3_3107 at 3728.029 kb on - strand, within Shewana3_3107 at 3728.037 kb on + strand, within Shewana3_3107 at 3728.042 kb on + strand, within Shewana3_3107 at 3728.137 kb on - strand at 3728.196 kb on + strand at 3728.379 kb on - strand, within Shewana3_3108 at 3728.504 kb on + strand, within Shewana3_3108 at 3728.512 kb on - strand, within Shewana3_3108 at 3728.557 kb on - strand, within Shewana3_3108 at 3729.080 kb on - strand, within Shewana3_3109 at 3729.086 kb on - strand, within Shewana3_3109 at 3729.212 kb on + strand, within Shewana3_3109
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 3,725,787 + Shewana3_3106 0.33 +0.0 3,725,797 + Shewana3_3106 0.33 +0.2 3,725,883 + Shewana3_3106 0.40 +0.2 3,725,912 - Shewana3_3106 0.42 -1.0 3,725,969 + Shewana3_3106 0.46 -1.2 3,725,970 + Shewana3_3106 0.46 -1.2 3,725,990 + Shewana3_3106 0.48 -1.6 3,725,990 + Shewana3_3106 0.48 +0.3 3,726,291 + Shewana3_3106 0.70 -0.5 3,726,318 + Shewana3_3106 0.72 -1.7 3,726,413 - Shewana3_3106 0.79 +1.1 3,726,413 - Shewana3_3106 0.79 -0.2 3,726,561 + +0.4 3,726,561 + -1.5 3,726,659 + +0.1 3,726,996 + Shewana3_3107 0.17 -1.5 3,727,047 - Shewana3_3107 0.20 +0.3 3,727,252 - Shewana3_3107 0.34 +0.2 3,727,311 + Shewana3_3107 0.38 +0.6 3,727,364 + Shewana3_3107 0.42 -1.3 3,727,437 + Shewana3_3107 0.47 -1.3 3,727,555 + Shewana3_3107 0.55 +0.2 3,727,563 - Shewana3_3107 0.55 +0.4 3,727,573 - Shewana3_3107 0.56 -0.9 3,727,851 + Shewana3_3107 0.74 -1.5 3,727,900 - Shewana3_3107 0.78 +0.5 3,727,929 - Shewana3_3107 0.80 +0.0 3,727,931 + Shewana3_3107 0.80 +0.4 3,728,018 + Shewana3_3107 0.86 +0.2 3,728,023 + Shewana3_3107 0.86 +0.3 3,728,029 - Shewana3_3107 0.86 +0.2 3,728,037 + Shewana3_3107 0.87 +0.4 3,728,042 + Shewana3_3107 0.87 +0.1 3,728,137 - +0.4 3,728,196 + -0.2 3,728,379 - Shewana3_3108 0.34 +0.5 3,728,504 + Shewana3_3108 0.69 +0.3 3,728,512 - Shewana3_3108 0.71 -0.3 3,728,557 - Shewana3_3108 0.84 +0.2 3,729,080 - Shewana3_3109 0.39 -1.2 3,729,086 - Shewana3_3109 0.39 -4.5 3,729,212 + Shewana3_3109 0.57 -1.6
Or see this region's nucleotide sequence