Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2560 and Shewana3_2561 are separated by 9 nucleotides Shewana3_2561 and rpmF are separated by 15 nucleotides rpmF and Shewana3_2563 are separated by 17 nucleotides
Shewana3_2560: Shewana3_2560 - 3-oxoacyl-(acyl carrier protein) synthase III (RefSeq), at 3,062,171 to 3,063,130
_2560
Shewana3_2561: Shewana3_2561 - putative glycerol-3-phosphate acyltransferase PlsX (RefSeq), at 3,063,140 to 3,064,168
_2561
Shewana3_2562: rpmF - 50S ribosomal protein L32 (RefSeq), at 3,064,184 to 3,064,354
rpmF
Shewana3_2563: Shewana3_2563 - hypothetical protein (RefSeq), at 3,064,372 to 3,064,896
_2563
Position (kb)
3063
3064
3065 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3062.309 kb on - strand, within Shewana3_2560 at 3062.652 kb on - strand, within Shewana3_2560 at 3062.696 kb on - strand at 3062.785 kb on - strand, within Shewana3_2560 at 3062.955 kb on - strand, within Shewana3_2560 at 3063.091 kb on - strand at 3065.041 kb on + strand at 3065.041 kb on + strand at 3065.069 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 3,062,309 - Shewana3_2560 0.14 -5.0 3,062,652 - Shewana3_2560 0.50 -2.3 3,062,696 - -0.8 3,062,785 - Shewana3_2560 0.64 -4.5 3,062,955 - Shewana3_2560 0.82 -2.5 3,063,091 - -3.7 3,065,041 + -1.6 3,065,041 + -0.6 3,065,069 + +0.6
Or see this region's nucleotide sequence