Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2272 and Shewana3_2273 are separated by 584 nucleotides Shewana3_2273 and Shewana3_2274 are separated by 68 nucleotides Shewana3_2274 and Shewana3_2275 are separated by 155 nucleotides Shewana3_2275 and hscA are separated by 8 nucleotides
Shewana3_2272: Shewana3_2272 - nucleoside diphosphate kinase (RefSeq), at 2,736,146 to 2,736,577
_2272
Shewana3_2273: Shewana3_2273 - hypothetical protein (RefSeq), at 2,737,162 to 2,737,359
_2273
Shewana3_2274: Shewana3_2274 - SSU ribosomal protein S6P modification protein (RefSeq), at 2,737,428 to 2,738,327
_2274
Shewana3_2275: Shewana3_2275 - ferredoxin, 2Fe-2S type, ISC system (RefSeq), at 2,738,483 to 2,738,818
_2275
Shewana3_2276: hscA - chaperone protein HscA (RefSeq), at 2,738,827 to 2,740,689
hscA
Position (kb)
2737
2738
2739 Strain fitness (log2 ratio)
-2
-1
0
1 at 2736.908 kb on + strand at 2737.404 kb on + strand at 2737.691 kb on - strand, within Shewana3_2274 at 2737.691 kb on - strand, within Shewana3_2274 at 2737.789 kb on + strand, within Shewana3_2274 at 2738.099 kb on + strand, within Shewana3_2274 at 2738.099 kb on + strand, within Shewana3_2274 at 2738.294 kb on + strand at 2738.302 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 2,736,908 + -1.8 2,737,404 + -0.7 2,737,691 - Shewana3_2274 0.29 -0.6 2,737,691 - Shewana3_2274 0.29 -1.2 2,737,789 + Shewana3_2274 0.40 -0.2 2,738,099 + Shewana3_2274 0.75 +0.2 2,738,099 + Shewana3_2274 0.75 -1.3 2,738,294 + +0.5 2,738,302 - -0.2
Or see this region's nucleotide sequence