Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_R0095 and Shewana3_R0096 are separated by 47 nucleotides Shewana3_R0096 and Shewana3_R0097 are separated by 31 nucleotides Shewana3_R0097 and Shewana3_R0098 are separated by 46 nucleotides Shewana3_R0098 and Shewana3_R0099 are separated by 46 nucleotides Shewana3_R0099 and Shewana3_2167 are separated by 208 nucleotides Shewana3_2167 and Shewana3_2168 are separated by 125 nucleotides Shewana3_2168 and Shewana3_2169 are separated by 0 nucleotides
Shewana3_R0095: Shewana3_R0095 - tRNA-Asn (RefSeq), at 2,597,882 to 2,597,957
_R0095
Shewana3_R0096: Shewana3_R0096 - tRNA-Asn (RefSeq), at 2,598,005 to 2,598,080
_R0096
Shewana3_R0097: Shewana3_R0097 - tRNA-Asn (RefSeq), at 2,598,112 to 2,598,187
_R0097
Shewana3_R0098: Shewana3_R0098 - tRNA-Asn (RefSeq), at 2,598,234 to 2,598,309
_R0098
Shewana3_R0099: Shewana3_R0099 - tRNA-Asn (RefSeq), at 2,598,356 to 2,598,431
_R0099
Shewana3_2167: Shewana3_2167 - diguanylate cyclase/phosphodiesterase (RefSeq), at 2,598,640 to 2,600,544
_2167
Shewana3_2168: Shewana3_2168 - Na(+)-translocating NADH-quinone reductase subunit E (RefSeq), at 2,600,670 to 2,601,248
_2168
Shewana3_2169: Shewana3_2169 - electron transport complex protein RnfB (RefSeq), at 2,601,249 to 2,601,830
_2169
Position (kb)
2598
2599
2600
2601 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2598.296 kb on + strand, within Shewana3_R0098 at 2598.472 kb on - strand at 2598.887 kb on - strand, within Shewana3_2167 at 2599.181 kb on + strand, within Shewana3_2167 at 2599.336 kb on + strand, within Shewana3_2167 at 2599.409 kb on - strand, within Shewana3_2167 at 2599.521 kb on + strand, within Shewana3_2167 at 2599.694 kb on + strand, within Shewana3_2167 at 2599.709 kb on + strand, within Shewana3_2167 at 2599.743 kb on - strand, within Shewana3_2167 at 2599.743 kb on - strand, within Shewana3_2167 at 2599.748 kb on - strand, within Shewana3_2167 at 2600.024 kb on + strand, within Shewana3_2167 at 2600.096 kb on + strand, within Shewana3_2167 at 2600.104 kb on - strand, within Shewana3_2167 at 2600.258 kb on - strand, within Shewana3_2167 at 2600.275 kb on + strand, within Shewana3_2167 at 2600.334 kb on + strand, within Shewana3_2167 at 2600.361 kb on - strand at 2600.511 kb on + strand at 2600.563 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 2,598,296 + Shewana3_R0098 0.82 -0.1 2,598,472 - -0.3 2,598,887 - Shewana3_2167 0.13 +0.7 2,599,181 + Shewana3_2167 0.28 +0.9 2,599,336 + Shewana3_2167 0.37 -3.5 2,599,409 - Shewana3_2167 0.40 +0.5 2,599,521 + Shewana3_2167 0.46 +0.4 2,599,694 + Shewana3_2167 0.55 -0.5 2,599,709 + Shewana3_2167 0.56 -1.5 2,599,743 - Shewana3_2167 0.58 +0.2 2,599,743 - Shewana3_2167 0.58 +0.3 2,599,748 - Shewana3_2167 0.58 -1.7 2,600,024 + Shewana3_2167 0.73 +0.2 2,600,096 + Shewana3_2167 0.76 +0.6 2,600,104 - Shewana3_2167 0.77 +0.2 2,600,258 - Shewana3_2167 0.85 +0.5 2,600,275 + Shewana3_2167 0.86 -0.3 2,600,334 + Shewana3_2167 0.89 -1.1 2,600,361 - +0.4 2,600,511 + -2.7 2,600,563 - -0.1
Or see this region's nucleotide sequence