Strain Fitness in Shewanella sp. ANA-3 around Shewana3_1848

Experiment: L-Asparagine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntShewana3_1847 and Shewana3_1848 are separated by 44 nucleotidesShewana3_1848 and Shewana3_1849 overlap by 19 nucleotidesShewana3_1849 and hisH are separated by 29 nucleotides Shewana3_1847: Shewana3_1847 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein (RefSeq), at 2,189,352 to 2,190,011 _1847 Shewana3_1848: Shewana3_1848 - imidazole glycerol phosphate synthase subunit HisF (RefSeq), at 2,190,056 to 2,190,829 _1848 Shewana3_1849: Shewana3_1849 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (RefSeq), at 2,190,811 to 2,191,548 _1849 Shewana3_1850: hisH - imidazole glycerol phosphate synthase subunit HisH (RefSeq), at 2,191,578 to 2,192,228 hisH Position (kb) 2190 2191Strain fitness (log2 ratio) -7 -6 -5 -4 -3 -2 -1 0 1at 2189.292 kb on + strandat 2189.292 kb on + strandat 2189.332 kb on - strandat 2189.337 kb on - strandat 2189.337 kb on - strandat 2189.339 kb on - strandat 2189.575 kb on + strand, within Shewana3_1847at 2189.640 kb on + strand, within Shewana3_1847at 2189.642 kb on + strand, within Shewana3_1847at 2189.996 kb on - strandat 2190.055 kb on - strandat 2190.406 kb on + strand, within Shewana3_1848at 2190.633 kb on + strand, within Shewana3_1848at 2190.634 kb on + strand, within Shewana3_1848at 2190.641 kb on - strand, within Shewana3_1848at 2190.778 kb on - strandat 2190.826 kb on + strandat 2190.981 kb on + strand, within Shewana3_1849at 2190.989 kb on - strand, within Shewana3_1849at 2191.094 kb on - strand, within Shewana3_1849at 2191.651 kb on + strand, within hisHat 2191.714 kb on - strand, within hisH

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Asparagine (N)
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2,189,292 + -1.1
2,189,292 + +0.9
2,189,332 - -0.8
2,189,337 - -3.8
2,189,337 - -1.5
2,189,339 - -0.1
2,189,575 + Shewana3_1847 0.34 -5.1
2,189,640 + Shewana3_1847 0.44 -6.4
2,189,642 + Shewana3_1847 0.44 -5.6
2,189,996 - -7.5
2,190,055 - -4.9
2,190,406 + Shewana3_1848 0.45 -7.1
2,190,633 + Shewana3_1848 0.75 -5.1
2,190,634 + Shewana3_1848 0.75 -4.4
2,190,641 - Shewana3_1848 0.76 -6.0
2,190,778 - -6.4
2,190,826 + -4.2
2,190,981 + Shewana3_1849 0.23 -5.7
2,190,989 - Shewana3_1849 0.24 -4.4
2,191,094 - Shewana3_1849 0.38 -6.8
2,191,651 + hisH Shewana3_1850 0.11 -3.5
2,191,714 - hisH Shewana3_1850 0.21 -6.8

Or see this region's nucleotide sequence