Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1583 and Shewana3_R0069 are separated by 543 nucleotides Shewana3_R0069 and Shewana3_R0070 are separated by 57 nucleotides Shewana3_R0070 and Shewana3_R0071 are separated by 45 nucleotides Shewana3_R0071 and Shewana3_R0072 are separated by 272 nucleotides
Shewana3_1583: Shewana3_1583 - D,D-heptose 1,7-bisphosphate phosphatase (RefSeq), at 1,852,270 to 1,852,821
_1583
Shewana3_R0069: Shewana3_R0069 - 16S ribosomal RNA (RefSeq), at 1,853,365 to 1,854,919
_R0069
Shewana3_R0070: Shewana3_R0070 - tRNA-Ile (RefSeq), at 1,854,977 to 1,855,053
_R0070
Shewana3_R0071: Shewana3_R0071 - tRNA-Ala (RefSeq), at 1,855,099 to 1,855,174
_R0071
Shewana3_R0072: Shewana3_R0072 - 23S ribosomal RNA (RefSeq), at 1,855,447 to 1,858,341
_R0072
Position (kb) 1853
1854
1855 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1852.583 kb on - strand, within Shewana3_1583 at 1852.661 kb on + strand, within Shewana3_1583 at 1852.686 kb on + strand, within Shewana3_1583 at 1852.741 kb on + strand, within Shewana3_1583 at 1852.749 kb on - strand, within Shewana3_1583 at 1852.948 kb on + strand at 1852.948 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 1,852,583 - Shewana3_1583 0.57 -2.4 1,852,661 + Shewana3_1583 0.71 -3.7 1,852,686 + Shewana3_1583 0.75 -2.4 1,852,741 + Shewana3_1583 0.85 -4.2 1,852,749 - Shewana3_1583 0.87 -0.9 1,852,948 + +1.0 1,852,948 + -0.5
Or see this region's nucleotide sequence