Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt infB and rbfA are separated by 62 nucleotides rbfA and Shewana3_1032 overlap by 1 nucleotides Shewana3_1032 and rpsO are separated by 126 nucleotides rpsO and Shewana3_1034 are separated by 86 nucleotides
Shewana3_1030: infB - translation initiation factor IF-2 (RefSeq), at 1,202,214 to 1,204,883
infB
Shewana3_1031: rbfA - ribosome-binding factor A (RefSeq), at 1,204,946 to 1,205,386
rbfA
Shewana3_1032: Shewana3_1032 - tRNA pseudouridine synthase B (RefSeq), at 1,205,386 to 1,206,339
_1032
Shewana3_1033: rpsO - 30S ribosomal protein S15 (RefSeq), at 1,206,466 to 1,206,735
rpsO
Shewana3_1034: Shewana3_1034 - diguanylate cyclase/phosphodiesterase (RefSeq), at 1,206,822 to 1,208,846
_1034
Position (kb) 1205
1206
1207 Strain fitness (log2 ratio) -2
-1
0
1 at 1205.293 kb on - strand, within rbfA at 1205.358 kb on + strand at 1205.667 kb on + strand, within Shewana3_1032 at 1206.307 kb on + strand at 1206.889 kb on + strand at 1207.138 kb on + strand, within Shewana3_1034 at 1207.192 kb on - strand, within Shewana3_1034
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 1,205,293 - rbfA Shewana3_1031 0.79 -2.2 1,205,358 + +0.2 1,205,667 + Shewana3_1032 0.29 -0.8 1,206,307 + +0.1 1,206,889 + -0.5 1,207,138 + Shewana3_1034 0.16 +0.4 1,207,192 - Shewana3_1034 0.18 -0.3
Or see this region's nucleotide sequence