Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0798 and Shewana3_0799 are separated by 62 nucleotides Shewana3_0799 and Shewana3_0800 are separated by 187 nucleotides Shewana3_0800 and Shewana3_0801 are separated by 142 nucleotides Shewana3_0801 and Shewana3_0802 are separated by 25 nucleotides
Shewana3_0798: Shewana3_0798 - DNA-binding transcriptional regulator AsnC (RefSeq), at 921,368 to 921,862
_0798
Shewana3_0799: Shewana3_0799 - ribosomal large subunit pseudouridine synthase A (RefSeq), at 921,925 to 922,602
_0799
Shewana3_0800: Shewana3_0800 - peptidase U32 (RefSeq), at 922,790 to 924,706
_0800
Shewana3_0801: Shewana3_0801 - hypothetical protein (RefSeq), at 924,849 to 925,223
_0801
Shewana3_0802: Shewana3_0802 - abortive infection protein (RefSeq), at 925,249 to 926,001
_0802
Position (kb) 922
923
924
925 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 921.928 kb on + strand at 921.984 kb on - strand at 922.008 kb on + strand, within Shewana3_0799 at 922.141 kb on - strand, within Shewana3_0799 at 922.153 kb on - strand, within Shewana3_0799 at 922.153 kb on - strand, within Shewana3_0799 at 922.182 kb on - strand, within Shewana3_0799 at 922.182 kb on - strand, within Shewana3_0799 at 922.182 kb on - strand, within Shewana3_0799 at 922.230 kb on + strand, within Shewana3_0799 at 922.471 kb on + strand, within Shewana3_0799 at 922.755 kb on - strand at 922.883 kb on + strand at 922.981 kb on + strand at 923.037 kb on - strand, within Shewana3_0800 at 923.119 kb on + strand, within Shewana3_0800 at 923.192 kb on - strand, within Shewana3_0800 at 923.443 kb on - strand, within Shewana3_0800 at 923.551 kb on - strand, within Shewana3_0800 at 923.655 kb on + strand, within Shewana3_0800 at 923.990 kb on + strand, within Shewana3_0800 at 923.998 kb on - strand, within Shewana3_0800 at 924.137 kb on + strand, within Shewana3_0800 at 924.265 kb on + strand, within Shewana3_0800 at 924.273 kb on - strand, within Shewana3_0800 at 924.273 kb on - strand, within Shewana3_0800 at 924.273 kb on - strand, within Shewana3_0800 at 924.527 kb on + strand at 924.591 kb on + strand at 924.591 kb on + strand at 924.591 kb on + strand at 924.943 kb on + strand, within Shewana3_0801 at 925.394 kb on + strand, within Shewana3_0802 at 925.402 kb on - strand, within Shewana3_0802 at 925.402 kb on - strand, within Shewana3_0802 at 925.402 kb on - strand, within Shewana3_0802 at 925.402 kb on - strand, within Shewana3_0802 at 925.404 kb on - strand, within Shewana3_0802 at 925.404 kb on - strand, within Shewana3_0802 at 925.404 kb on - strand, within Shewana3_0802 at 925.515 kb on - strand, within Shewana3_0802 at 925.555 kb on + strand, within Shewana3_0802 at 925.563 kb on - strand, within Shewana3_0802 at 925.638 kb on - strand, within Shewana3_0802 at 925.689 kb on - strand, within Shewana3_0802
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 921,928 + +0.4 921,984 - +0.3 922,008 + Shewana3_0799 0.12 +0.2 922,141 - Shewana3_0799 0.32 +0.1 922,153 - Shewana3_0799 0.34 +0.5 922,153 - Shewana3_0799 0.34 +0.3 922,182 - Shewana3_0799 0.38 +0.5 922,182 - Shewana3_0799 0.38 +0.1 922,182 - Shewana3_0799 0.38 +0.8 922,230 + Shewana3_0799 0.45 -0.7 922,471 + Shewana3_0799 0.81 -1.1 922,755 - +0.1 922,883 + -0.0 922,981 + +0.1 923,037 - Shewana3_0800 0.13 -4.1 923,119 + Shewana3_0800 0.17 -1.3 923,192 - Shewana3_0800 0.21 +0.8 923,443 - Shewana3_0800 0.34 -0.5 923,551 - Shewana3_0800 0.40 -2.1 923,655 + Shewana3_0800 0.45 -1.2 923,990 + Shewana3_0800 0.63 -1.6 923,998 - Shewana3_0800 0.63 +0.1 924,137 + Shewana3_0800 0.70 -1.4 924,265 + Shewana3_0800 0.77 -1.4 924,273 - Shewana3_0800 0.77 -1.6 924,273 - Shewana3_0800 0.77 -0.7 924,273 - Shewana3_0800 0.77 -1.5 924,527 + +0.1 924,591 + +0.0 924,591 + -0.4 924,591 + -1.6 924,943 + Shewana3_0801 0.25 +0.8 925,394 + Shewana3_0802 0.19 +0.6 925,402 - Shewana3_0802 0.20 +0.3 925,402 - Shewana3_0802 0.20 -1.2 925,402 - Shewana3_0802 0.20 +0.4 925,402 - Shewana3_0802 0.20 +0.4 925,404 - Shewana3_0802 0.21 +0.3 925,404 - Shewana3_0802 0.21 -1.4 925,404 - Shewana3_0802 0.21 -1.7 925,515 - Shewana3_0802 0.35 +0.0 925,555 + Shewana3_0802 0.41 +0.5 925,563 - Shewana3_0802 0.42 -1.1 925,638 - Shewana3_0802 0.52 +0.2 925,689 - Shewana3_0802 0.58 -1.3
Or see this region's nucleotide sequence