Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0440 and purH are separated by 105 nucleotides purH and zntR are separated by 336 nucleotides zntR and Shewana3_0443 are separated by 2 nucleotides
Shewana3_0440: Shewana3_0440 - phosphoribosylamine--glycine ligase (RefSeq), at 514,383 to 515,681
_0440
Shewana3_0441: purH - bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (RefSeq), at 515,787 to 517,385
purH
Shewana3_0442: zntR - zinc-responsive transcriptional regulator (RefSeq), at 517,722 to 518,195
zntR
Shewana3_0443: Shewana3_0443 - permease (RefSeq), at 518,198 to 519,691
_0443
Position (kb) 515
516
517
518 Strain fitness (log2 ratio) -8
-7
-6
-5
-4
-3
-2
-1
0
1
2 at 514.845 kb on + strand, within Shewana3_0440 at 514.853 kb on - strand, within Shewana3_0440 at 514.919 kb on + strand, within Shewana3_0440 at 514.999 kb on - strand, within Shewana3_0440 at 515.103 kb on - strand, within Shewana3_0440 at 515.209 kb on + strand, within Shewana3_0440 at 515.283 kb on + strand, within Shewana3_0440 at 515.291 kb on - strand, within Shewana3_0440 at 515.318 kb on - strand, within Shewana3_0440 at 515.352 kb on - strand, within Shewana3_0440 at 515.366 kb on + strand, within Shewana3_0440 at 515.374 kb on - strand, within Shewana3_0440 at 515.374 kb on - strand, within Shewana3_0440 at 515.425 kb on + strand, within Shewana3_0440 at 515.433 kb on - strand, within Shewana3_0440 at 515.462 kb on + strand, within Shewana3_0440 at 515.516 kb on + strand, within Shewana3_0440 at 515.516 kb on + strand, within Shewana3_0440 at 515.524 kb on - strand, within Shewana3_0440 at 515.524 kb on - strand, within Shewana3_0440 at 515.593 kb on + strand at 515.593 kb on + strand at 515.596 kb on - strand at 515.601 kb on - strand at 515.603 kb on + strand at 515.736 kb on - strand at 515.956 kb on - strand, within purH at 515.983 kb on - strand, within purH at 515.983 kb on - strand, within purH at 515.983 kb on - strand, within purH at 516.165 kb on - strand, within purH at 516.169 kb on + strand, within purH at 516.169 kb on + strand, within purH at 516.169 kb on + strand, within purH at 516.178 kb on - strand, within purH at 516.258 kb on - strand, within purH at 516.388 kb on - strand, within purH at 516.481 kb on + strand, within purH at 516.481 kb on + strand, within purH at 516.491 kb on + strand, within purH at 516.499 kb on + strand, within purH at 516.499 kb on - strand, within purH at 516.499 kb on - strand, within purH at 516.499 kb on - strand, within purH at 516.556 kb on - strand, within purH at 516.566 kb on - strand, within purH at 516.599 kb on + strand, within purH at 516.774 kb on - strand, within purH at 516.782 kb on - strand, within purH at 517.056 kb on + strand, within purH at 517.064 kb on + strand, within purH at 517.154 kb on + strand, within purH at 517.176 kb on - strand, within purH at 517.226 kb on + strand at 517.385 kb on + strand at 517.566 kb on + strand at 517.640 kb on + strand at 517.648 kb on - strand at 517.679 kb on + strand at 517.713 kb on + strand at 517.714 kb on + strand at 517.779 kb on - strand, within zntR at 517.807 kb on - strand, within zntR at 517.835 kb on + strand, within zntR at 517.843 kb on - strand, within zntR at 517.952 kb on + strand, within zntR at 518.115 kb on - strand, within zntR at 518.147 kb on - strand, within zntR at 518.245 kb on + strand at 518.375 kb on - strand, within Shewana3_0443
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 514,845 + Shewana3_0440 0.36 -5.5 514,853 - Shewana3_0440 0.36 -4.2 514,919 + Shewana3_0440 0.41 -6.8 514,999 - Shewana3_0440 0.47 -5.6 515,103 - Shewana3_0440 0.55 -6.6 515,209 + Shewana3_0440 0.64 -3.7 515,283 + Shewana3_0440 0.69 -4.5 515,291 - Shewana3_0440 0.70 -4.7 515,318 - Shewana3_0440 0.72 -3.8 515,352 - Shewana3_0440 0.75 -5.6 515,366 + Shewana3_0440 0.76 -7.2 515,374 - Shewana3_0440 0.76 -5.0 515,374 - Shewana3_0440 0.76 -6.1 515,425 + Shewana3_0440 0.80 -5.5 515,433 - Shewana3_0440 0.81 -5.5 515,462 + Shewana3_0440 0.83 -3.9 515,516 + Shewana3_0440 0.87 -1.8 515,516 + Shewana3_0440 0.87 -7.2 515,524 - Shewana3_0440 0.88 -1.6 515,524 - Shewana3_0440 0.88 -5.1 515,593 + -6.2 515,593 + -5.5 515,596 - -5.6 515,601 - -5.8 515,603 + -4.8 515,736 - -1.2 515,956 - purH Shewana3_0441 0.11 -5.4 515,983 - purH Shewana3_0441 0.12 -4.2 515,983 - purH Shewana3_0441 0.12 -5.9 515,983 - purH Shewana3_0441 0.12 -5.6 516,165 - purH Shewana3_0441 0.24 -6.3 516,169 + purH Shewana3_0441 0.24 -5.8 516,169 + purH Shewana3_0441 0.24 -3.8 516,169 + purH Shewana3_0441 0.24 -6.5 516,178 - purH Shewana3_0441 0.24 -6.3 516,258 - purH Shewana3_0441 0.29 -6.1 516,388 - purH Shewana3_0441 0.38 -5.4 516,481 + purH Shewana3_0441 0.43 -6.3 516,481 + purH Shewana3_0441 0.43 -6.0 516,491 + purH Shewana3_0441 0.44 -5.3 516,499 + purH Shewana3_0441 0.45 -6.3 516,499 - purH Shewana3_0441 0.45 -5.5 516,499 - purH Shewana3_0441 0.45 -8.0 516,499 - purH Shewana3_0441 0.45 -5.6 516,556 - purH Shewana3_0441 0.48 -6.5 516,566 - purH Shewana3_0441 0.49 -6.3 516,599 + purH Shewana3_0441 0.51 -3.9 516,774 - purH Shewana3_0441 0.62 -5.0 516,782 - purH Shewana3_0441 0.62 -5.3 517,056 + purH Shewana3_0441 0.79 -4.2 517,064 + purH Shewana3_0441 0.80 -6.1 517,154 + purH Shewana3_0441 0.85 -5.3 517,176 - purH Shewana3_0441 0.87 -3.7 517,226 + -5.2 517,385 + -7.2 517,566 + -0.5 517,640 + +0.6 517,648 - -0.7 517,679 + +0.4 517,713 + -0.9 517,714 + +0.4 517,779 - zntR Shewana3_0442 0.12 -0.6 517,807 - zntR Shewana3_0442 0.18 +2.0 517,835 + zntR Shewana3_0442 0.24 +0.1 517,843 - zntR Shewana3_0442 0.26 -0.7 517,952 + zntR Shewana3_0442 0.49 -0.7 518,115 - zntR Shewana3_0442 0.83 -0.6 518,147 - zntR Shewana3_0442 0.90 +0.6 518,245 + +0.8 518,375 - Shewana3_0443 0.12 +1.0
Or see this region's nucleotide sequence