Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0253 and Shewana3_0254 are separated by 302 nucleotides Shewana3_0254 and Shewana3_0255 are separated by 50 nucleotides Shewana3_0255 and Shewana3_0256 are separated by 38 nucleotides
Shewana3_0253: Shewana3_0253 - two component transcriptional regulator (RefSeq), at 285,338 to 286,024
_0253
Shewana3_0254: Shewana3_0254 - protein of unknown function, Spy-related (RefSeq), at 286,327 to 286,818
_0254
Shewana3_0255: Shewana3_0255 - cation diffusion facilitator family transporter (RefSeq), at 286,869 to 287,768
_0255
Shewana3_0256: Shewana3_0256 - hypothetical protein (RefSeq), at 287,807 to 288,217
_0256
Position (kb) 286
287 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1
2
3 at 285.498 kb on - strand, within Shewana3_0253 at 285.794 kb on + strand, within Shewana3_0253 at 285.863 kb on + strand, within Shewana3_0253 at 285.907 kb on + strand, within Shewana3_0253 at 285.907 kb on + strand, within Shewana3_0253 at 285.907 kb on + strand, within Shewana3_0253 at 285.915 kb on - strand, within Shewana3_0253 at 285.915 kb on - strand, within Shewana3_0253 at 285.915 kb on - strand, within Shewana3_0253 at 285.915 kb on - strand, within Shewana3_0253 at 285.927 kb on + strand, within Shewana3_0253 at 285.943 kb on + strand, within Shewana3_0253 at 286.049 kb on + strand at 286.058 kb on + strand at 286.125 kb on + strand at 286.195 kb on - strand at 286.226 kb on - strand at 286.282 kb on - strand at 286.286 kb on + strand at 286.434 kb on + strand, within Shewana3_0254 at 286.676 kb on + strand, within Shewana3_0254 at 286.751 kb on + strand, within Shewana3_0254 at 286.751 kb on + strand, within Shewana3_0254 at 286.812 kb on + strand at 286.850 kb on - strand at 286.947 kb on - strand at 286.967 kb on + strand, within Shewana3_0255 at 287.034 kb on + strand, within Shewana3_0255 at 287.107 kb on - strand, within Shewana3_0255 at 287.486 kb on - strand, within Shewana3_0255 at 287.494 kb on + strand, within Shewana3_0255 at 287.502 kb on - strand, within Shewana3_0255 at 287.589 kb on - strand, within Shewana3_0255 at 287.599 kb on - strand, within Shewana3_0255 at 287.719 kb on - strand at 287.744 kb on + strand at 287.744 kb on + strand at 287.809 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 285,498 - Shewana3_0253 0.23 -0.2 285,794 + Shewana3_0253 0.66 -0.1 285,863 + Shewana3_0253 0.76 +0.4 285,907 + Shewana3_0253 0.83 -1.3 285,907 + Shewana3_0253 0.83 +0.4 285,907 + Shewana3_0253 0.83 +0.2 285,915 - Shewana3_0253 0.84 -0.4 285,915 - Shewana3_0253 0.84 +3.3 285,915 - Shewana3_0253 0.84 +0.0 285,915 - Shewana3_0253 0.84 +0.1 285,927 + Shewana3_0253 0.86 -1.5 285,943 + Shewana3_0253 0.88 -0.1 286,049 + +0.0 286,058 + -1.0 286,125 + -0.2 286,195 - -1.2 286,226 - +0.1 286,282 - +0.4 286,286 + +0.4 286,434 + Shewana3_0254 0.22 -1.0 286,676 + Shewana3_0254 0.71 -1.1 286,751 + Shewana3_0254 0.86 +0.5 286,751 + Shewana3_0254 0.86 +0.3 286,812 + -2.2 286,850 - -5.6 286,947 - -5.2 286,967 + Shewana3_0255 0.11 -6.8 287,034 + Shewana3_0255 0.18 -3.4 287,107 - Shewana3_0255 0.26 -5.4 287,486 - Shewana3_0255 0.69 -2.1 287,494 + Shewana3_0255 0.69 -6.5 287,502 - Shewana3_0255 0.70 -4.2 287,589 - Shewana3_0255 0.80 -4.7 287,599 - Shewana3_0255 0.81 -4.3 287,719 - -5.5 287,744 + -1.1 287,744 + -1.0 287,809 + -0.9
Or see this region's nucleotide sequence