Experiment: acetate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt alaS and Sama_1049 are separated by 33 nucleotides Sama_1049 and Sama_1050 are separated by 113 nucleotides Sama_1050 and Sama_R0038 are separated by 230 nucleotides Sama_R0038 and Sama_R0039 are separated by 23 nucleotides Sama_R0039 and Sama_R0040 are separated by 35 nucleotides Sama_R0040 and Sama_R0041 are separated by 32 nucleotides Sama_R0041 and Sama_R0042 are separated by 39 nucleotides
Sama_1048: alaS - alanyl-tRNA synthetase (RefSeq), at 1,267,153 to 1,269,855
alaS
Sama_1049: Sama_1049 - aspartate kinase (RefSeq), at 1,269,889 to 1,271,100
_1049
Sama_1050: Sama_1050 - carbon storage regulator (RefSeq), at 1,271,214 to 1,271,408
_1050
Sama_R0038: Sama_R0038 - tRNA-Ser (RefSeq), at 1,271,639 to 1,271,730
_R0038
Sama_R0039: Sama_R0039 - tRNA-Arg (RefSeq), at 1,271,754 to 1,271,830
_R0039
Sama_R0040: Sama_R0040 - tRNA-Arg (RefSeq), at 1,271,866 to 1,271,942
_R0040
Sama_R0041: Sama_R0041 - tRNA-Arg (RefSeq), at 1,271,975 to 1,272,051
_R0041
Sama_R0042: Sama_R0042 - tRNA-Arg (RefSeq), at 1,272,091 to 1,272,167
_R0042
Position (kb)
1269
1270
1271
1272 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1269.873 kb on + strand at 1269.876 kb on + strand at 1269.876 kb on + strand at 1270.122 kb on + strand, within Sama_1049 at 1271.092 kb on - strand at 1271.102 kb on + strand at 1271.125 kb on + strand at 1271.153 kb on + strand at 1271.156 kb on + strand at 1271.403 kb on + strand at 1271.403 kb on + strand at 1271.403 kb on + strand at 1271.417 kb on + strand at 1271.418 kb on - strand at 1271.451 kb on - strand at 1271.451 kb on - strand at 1271.451 kb on - strand at 1271.488 kb on + strand at 1271.493 kb on + strand at 1271.527 kb on + strand at 1271.527 kb on + strand at 1271.528 kb on - strand at 1271.528 kb on - strand at 1271.528 kb on - strand at 1271.528 kb on - strand at 1271.958 kb on + strand at 1271.958 kb on + strand at 1272.056 kb on + strand at 1272.056 kb on + strand at 1272.056 kb on + strand at 1272.056 kb on + strand at 1272.068 kb on + strand at 1272.068 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction acetate remove 1,269,873 + -0.7 1,269,876 + +2.3 1,269,876 + -0.2 1,270,122 + Sama_1049 0.19 -1.6 1,271,092 - -0.7 1,271,102 + +1.6 1,271,125 + +0.1 1,271,153 + -0.3 1,271,156 + -0.1 1,271,403 + +0.2 1,271,403 + -0.3 1,271,403 + -0.2 1,271,417 + -1.8 1,271,418 - +0.0 1,271,451 - +0.2 1,271,451 - -1.2 1,271,451 - -0.2 1,271,488 + -1.4 1,271,493 + +0.3 1,271,527 + -0.5 1,271,527 + +0.6 1,271,528 - +1.4 1,271,528 - +0.3 1,271,528 - +1.1 1,271,528 - +0.1 1,271,958 + +0.0 1,271,958 + +0.3 1,272,056 + -1.0 1,272,056 + +0.3 1,272,056 + -0.8 1,272,056 + +0.8 1,272,068 + -0.2 1,272,068 + +0.6
Or see this region's nucleotide sequence