Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2466 and Shew_2467 are separated by 125 nucleotides Shew_2467 and Shew_2468 are separated by 32 nucleotides Shew_2468 and Shew_2469 are separated by 48 nucleotides Shew_2469 and Shew_2470 are separated by 4 nucleotides Shew_2470 and Shew_2471 are separated by 91 nucleotides
Shew_2466: Shew_2466 - hypothetical protein (RefSeq), at 2,902,171 to 2,904,141
_2466
Shew_2467: Shew_2467 - hypothetical protein (RefSeq), at 2,904,267 to 2,904,653
_2467
Shew_2468: Shew_2468 - RNA-binding S4 domain-containing protein (RefSeq), at 2,904,686 to 2,904,916
_2468
Shew_2469: Shew_2469 - GCN5-related N-acetyltransferase (RefSeq), at 2,904,965 to 2,905,513
_2469
Shew_2470: Shew_2470 - PpiC-type peptidyl-prolyl cis-trans isomerase (RefSeq), at 2,905,518 to 2,905,796
_2470
Shew_2471: Shew_2471 - hypothetical protein (RefSeq), at 2,905,888 to 2,907,723
_2471
Position (kb) 2904
2905
2906 Strain fitness (log2 ratio) -1
0
1 at 2904.157 kb on - strand at 2905.140 kb on + strand, within Shew_2469 at 2905.580 kb on - strand, within Shew_2470 at 2905.654 kb on + strand, within Shew_2470 at 2906.226 kb on - strand, within Shew_2471 at 2906.306 kb on + strand, within Shew_2471
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,904,157 - -0.2 2,905,140 + Shew_2469 0.32 -1.5 2,905,580 - Shew_2470 0.22 -0.4 2,905,654 + Shew_2470 0.49 -0.2 2,906,226 - Shew_2471 0.18 -0.3 2,906,306 + Shew_2471 0.23 -1.6
Or see this region's nucleotide sequence