Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1513 and upp are separated by 211 nucleotides upp and Shew_1515 are separated by 94 nucleotides Shew_1515 and Shew_1516 are separated by 313 nucleotides
Shew_1513: Shew_1513 - phosphoribosylaminoimidazole synthetase (RefSeq), at 1,735,736 to 1,736,773
_1513
Shew_1514: upp - uracil phosphoribosyltransferase (RefSeq), at 1,736,985 to 1,737,611
upp
Shew_1515: Shew_1515 - Na+/H+ antiporter NhaC (RefSeq), at 1,737,706 to 1,739,025
_1515
Shew_1516: Shew_1516 - alkyl hydroperoxide reductase/ Thiol specific antioxidant/ Mal allergen (RefSeq), at 1,739,339 to 1,739,944
_1516
Position (kb)
1737
1738
1739
1740 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1737.150 kb on + strand, within upp at 1737.570 kb on + strand at 1737.601 kb on + strand at 1737.699 kb on + strand at 1737.759 kb on + strand at 1737.767 kb on - strand at 1737.899 kb on - strand, within Shew_1515 at 1738.563 kb on + strand, within Shew_1515 at 1738.624 kb on + strand, within Shew_1515 at 1738.988 kb on - strand at 1739.083 kb on - strand at 1739.093 kb on - strand at 1739.164 kb on + strand at 1739.387 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,737,150 + upp Shew_1514 0.26 +0.1 1,737,570 + -0.5 1,737,601 + +1.8 1,737,699 + -0.6 1,737,759 + -4.0 1,737,767 - -0.5 1,737,899 - Shew_1515 0.15 +1.6 1,738,563 + Shew_1515 0.65 -0.8 1,738,624 + Shew_1515 0.70 -0.8 1,738,988 - -2.2 1,739,083 - +2.8 1,739,093 - +0.8 1,739,164 + +0.3 1,739,387 + +1.0
Or see this region's nucleotide sequence