Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0621 and Shew_0622 are separated by 60 nucleotides Shew_0622 and Shew_0623 are separated by 207 nucleotides Shew_0623 and Shew_0624 are separated by 70 nucleotides Shew_0624 and Shew_0625 are separated by 90 nucleotides
Shew_0621: Shew_0621 - hypothetical protein (RefSeq), at 706,643 to 706,975
_0621
Shew_0622: Shew_0622 - hypothetical protein (RefSeq), at 707,036 to 707,680
_0622
Shew_0623: Shew_0623 - phage shock protein A, PspA (RefSeq), at 707,888 to 708,574
_0623
Shew_0624: Shew_0624 - hypothetical protein (RefSeq), at 708,645 to 709,058
_0624
Shew_0625: Shew_0625 - spermidine synthase (RefSeq), at 709,149 to 710,876
_0625
Position (kb) 707
708
709 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 706.990 kb on - strand at 707.029 kb on + strand at 707.029 kb on + strand at 707.111 kb on - strand, within Shew_0622 at 707.495 kb on - strand, within Shew_0622 at 707.848 kb on + strand at 707.917 kb on - strand at 708.203 kb on - strand, within Shew_0623 at 708.685 kb on - strand at 709.233 kb on + strand at 709.289 kb on + strand at 709.289 kb on + strand at 709.289 kb on + strand at 709.297 kb on - strand at 709.300 kb on + strand at 709.448 kb on + strand, within Shew_0625 at 709.496 kb on - strand, within Shew_0625
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 706,990 - +0.5 707,029 + -0.9 707,029 + +0.9 707,111 - Shew_0622 0.12 -0.3 707,495 - Shew_0622 0.71 -3.0 707,848 + +0.9 707,917 - +0.3 708,203 - Shew_0623 0.46 -1.5 708,685 - +0.5 709,233 + +0.5 709,289 + +0.1 709,289 + -0.9 709,289 + -0.9 709,297 - -0.8 709,300 + -0.1 709,448 + Shew_0625 0.17 +0.1 709,496 - Shew_0625 0.20 -0.6
Or see this region's nucleotide sequence