Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0228 and rnpB are separated by 109 nucleotides rnpB and Shew_0229 are separated by 96 nucleotides Shew_0229 and Shew_0230 are separated by 69 nucleotides
Shew_0228: Shew_0228 - uroporphyrin-III C/tetrapyrrole methyltransferase (RefSeq), at 241,543 to 242,385
_0228
Shew_R0013: rnpB - RNA component of RNaseP (NCBI), at 242,495 to 242,800
rnpB
Shew_0229: Shew_0229 - extracellular solute-binding protein (RefSeq), at 242,897 to 243,706
_0229
Shew_0230: Shew_0230 - hypothetical protein (RefSeq), at 243,776 to 245,485
_0230
Position (kb) 242
243
244 Strain fitness (log2 ratio) -2
-1
0
1 at 241.921 kb on - strand, within Shew_0228 at 242.158 kb on - strand, within Shew_0228 at 242.233 kb on + strand, within Shew_0228 at 242.331 kb on - strand at 242.416 kb on + strand at 242.904 kb on + strand at 243.030 kb on - strand, within Shew_0229 at 243.590 kb on + strand, within Shew_0229 at 243.598 kb on - strand, within Shew_0229 at 243.762 kb on + strand at 243.770 kb on - strand at 243.845 kb on + strand at 243.858 kb on - strand at 243.867 kb on - strand at 244.107 kb on - strand, within Shew_0230 at 244.165 kb on + strand, within Shew_0230 at 244.173 kb on - strand, within Shew_0230 at 244.607 kb on + strand, within Shew_0230
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 241,921 - Shew_0228 0.45 -0.2 242,158 - Shew_0228 0.73 -2.6 242,233 + Shew_0228 0.82 -0.2 242,331 - -0.5 242,416 + -0.0 242,904 + -0.1 243,030 - Shew_0229 0.16 -0.4 243,590 + Shew_0229 0.86 -0.1 243,598 - Shew_0229 0.87 -1.7 243,762 + -0.3 243,770 - +0.0 243,845 + +0.2 243,858 - -0.0 243,867 - +0.3 244,107 - Shew_0230 0.19 -0.0 244,165 + Shew_0230 0.23 -0.2 244,173 - Shew_0230 0.23 -0.3 244,607 + Shew_0230 0.49 +0.1
Or see this region's nucleotide sequence