Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0140 and Shew_R0001 are separated by 631 nucleotides Shew_R0001 and Shew_R0002 are separated by 57 nucleotides Shew_R0002 and Shew_R0003 are separated by 52 nucleotides Shew_R0003 and Shew_R0004 are separated by 245 nucleotides
Shew_0140: Shew_0140 - RNP-1-like RNA-binding protein (RefSeq), at 149,997 to 150,455
_0140
Shew_R0001: Shew_R0001 - 16S ribosomal RNA (RefSeq), at 151,087 to 152,641
_R0001
Shew_R0002: Shew_R0002 - tRNA-Ile (RefSeq), at 152,699 to 152,775
_R0002
Shew_R0003: Shew_R0003 - tRNA-Ala (RefSeq), at 152,828 to 152,903
_R0003
Shew_R0004: Shew_R0004 - 23S ribosomal RNA (RefSeq), at 153,149 to 156,041
_R0004
Position (kb)
151
152
153 Strain fitness (log2 ratio)
-2
-1
0
1 at 150.135 kb on + strand, within Shew_0140 at 150.201 kb on - strand, within Shew_0140 at 150.238 kb on + strand, within Shew_0140 at 150.238 kb on + strand, within Shew_0140 at 150.238 kb on + strand, within Shew_0140 at 150.238 kb on + strand, within Shew_0140 at 150.238 kb on + strand, within Shew_0140 at 150.238 kb on + strand, within Shew_0140 at 150.246 kb on - strand, within Shew_0140 at 150.278 kb on + strand, within Shew_0140 at 150.280 kb on + strand, within Shew_0140 at 150.288 kb on - strand, within Shew_0140 at 150.318 kb on - strand, within Shew_0140 at 150.385 kb on + strand, within Shew_0140 at 150.393 kb on - strand, within Shew_0140 at 150.393 kb on - strand, within Shew_0140
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 150,135 + Shew_0140 0.30 -0.9 150,201 - Shew_0140 0.44 -0.3 150,238 + Shew_0140 0.53 -0.7 150,238 + Shew_0140 0.53 -1.2 150,238 + Shew_0140 0.53 -0.2 150,238 + Shew_0140 0.53 -0.2 150,238 + Shew_0140 0.53 -1.8 150,238 + Shew_0140 0.53 +0.0 150,246 - Shew_0140 0.54 -0.8 150,278 + Shew_0140 0.61 +0.1 150,280 + Shew_0140 0.62 -0.9 150,288 - Shew_0140 0.63 -0.5 150,318 - Shew_0140 0.70 -0.5 150,385 + Shew_0140 0.85 +0.2 150,393 - Shew_0140 0.86 -0.7 150,393 - Shew_0140 0.86 -1.1
Or see this region's nucleotide sequence