Experiment: copper (II) chloride 0.64 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nhaR and SO1339 are separated by 83 nucleotides SO1339 and SO1340 overlap by 20 nucleotides SO1340 and nadB are separated by 81 nucleotides nadB and rpoE are separated by 189 nucleotides rpoE and rseA are separated by 42 nucleotides
SO1338: nhaR - transcriptional activator protein NhaR (NCBI ptt file), at 1,395,632 to 1,396,540
nhaR
SO1339: SO1339 - conserved hypothetical protein (NCBI ptt file), at 1,396,624 to 1,396,872
SO1339
SO1340: SO1340 - hypothetical protein (NCBI ptt file), at 1,396,853 to 1,397,311
SO1340
SO1341: nadB - L-aspartate oxidase (NCBI ptt file), at 1,397,393 to 1,399,006
nadB
SO1342: rpoE - RNA polymerase sigma-24 factor (NCBI ptt file), at 1,399,196 to 1,399,774
rpoE
SO1343: rseA - sigma-E factor negative regulatory protein (NCBI ptt file), at 1,399,817 to 1,400,440
rseA
Position (kb)
1397
1398
1399
1400 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1396.409 kb on - strand, within nhaR at 1396.542 kb on - strand at 1396.575 kb on - strand at 1396.735 kb on - strand, within SO1339 at 1396.972 kb on + strand, within SO1340 at 1397.046 kb on + strand, within SO1340 at 1397.049 kb on - strand, within SO1340 at 1397.054 kb on - strand, within SO1340 at 1397.141 kb on + strand, within SO1340 at 1397.194 kb on + strand, within SO1340 at 1397.194 kb on - strand, within SO1340 at 1397.338 kb on + strand at 1397.832 kb on - strand, within nadB at 1398.136 kb on - strand, within nadB at 1398.357 kb on + strand, within nadB at 1398.367 kb on + strand, within nadB at 1398.435 kb on + strand, within nadB at 1398.443 kb on - strand, within nadB at 1398.581 kb on + strand, within nadB at 1398.589 kb on - strand, within nadB at 1398.868 kb on + strand at 1399.190 kb on + strand at 1399.190 kb on + strand at 1399.190 kb on + strand at 1399.190 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 0.64 mM remove 1,396,409 - nhaR SO1338 0.85 -0.8 1,396,542 - -0.1 1,396,575 - +0.4 1,396,735 - SO1339 0.45 -3.3 1,396,972 + SO1340 0.26 +0.1 1,397,046 + SO1340 0.42 +1.0 1,397,049 - SO1340 0.43 +0.6 1,397,054 - SO1340 0.44 -0.1 1,397,141 + SO1340 0.63 -0.1 1,397,194 + SO1340 0.74 -0.3 1,397,194 - SO1340 0.74 -0.2 1,397,338 + +0.2 1,397,832 - nadB SO1341 0.27 +0.3 1,398,136 - nadB SO1341 0.46 -0.8 1,398,357 + nadB SO1341 0.60 +0.2 1,398,367 + nadB SO1341 0.60 -1.1 1,398,435 + nadB SO1341 0.65 -0.8 1,398,443 - nadB SO1341 0.65 -1.1 1,398,581 + nadB SO1341 0.74 -2.1 1,398,589 - nadB SO1341 0.74 -3.3 1,398,868 + -0.4 1,399,190 + -0.9 1,399,190 + -0.3 1,399,190 + -0.1 1,399,190 + -1.4
Or see this region's nucleotide sequence