Experiment: Fumarate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_476 and HP15_477 overlap by 4 nucleotides HP15_477 and HP15_478 are separated by 65 nucleotides HP15_478 and HP15_479 are separated by 136 nucleotides HP15_479 and HP15_480 overlap by 8 nucleotides
HP15_476: HP15_476 - type 4 fimbrial biogenesis protein PilO, at 486,974 to 487,585
_476
HP15_477: HP15_477 - type 4 fimbrial biogenesis protein PilP, at 487,582 to 488,136
_477
HP15_478: HP15_478 - type 4 fimbrial biogenesis protein PilQ, at 488,202 to 490,268
_478
HP15_479: HP15_479 - shikimate kinase I, at 490,405 to 490,971
_479
HP15_480: HP15_480 - 3-dehydroquinate synthase, at 490,964 to 492,058
_480
Position (kb) 488
489
490
491 Strain fitness (log2 ratio) -2
-1
0
1 at 487.660 kb on - strand, within HP15_477 at 487.694 kb on - strand, within HP15_477 at 487.694 kb on - strand, within HP15_477 at 487.701 kb on - strand, within HP15_477 at 487.726 kb on - strand, within HP15_477 at 487.748 kb on - strand, within HP15_477 at 487.869 kb on + strand, within HP15_477 at 487.956 kb on + strand, within HP15_477 at 487.987 kb on + strand, within HP15_477 at 488.016 kb on - strand, within HP15_477 at 488.261 kb on + strand at 488.426 kb on - strand, within HP15_478 at 488.465 kb on - strand, within HP15_478 at 488.550 kb on - strand, within HP15_478 at 488.665 kb on + strand, within HP15_478 at 488.706 kb on - strand, within HP15_478 at 488.733 kb on - strand, within HP15_478 at 488.857 kb on - strand, within HP15_478 at 488.893 kb on + strand, within HP15_478 at 488.991 kb on + strand, within HP15_478 at 489.281 kb on + strand, within HP15_478 at 489.522 kb on + strand, within HP15_478 at 489.700 kb on + strand, within HP15_478 at 489.829 kb on - strand, within HP15_478 at 489.860 kb on - strand, within HP15_478 at 490.206 kb on - strand at 490.270 kb on - strand at 490.384 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fumarate remove 487,660 - HP15_477 0.14 +1.4 487,694 - HP15_477 0.20 -1.1 487,694 - HP15_477 0.20 -0.3 487,701 - HP15_477 0.21 -0.5 487,726 - HP15_477 0.26 -0.6 487,748 - HP15_477 0.30 -1.7 487,869 + HP15_477 0.52 +1.3 487,956 + HP15_477 0.67 +0.1 487,987 + HP15_477 0.73 -0.8 488,016 - HP15_477 0.78 +1.7 488,261 + +0.3 488,426 - HP15_478 0.11 -1.3 488,465 - HP15_478 0.13 -0.7 488,550 - HP15_478 0.17 -1.2 488,665 + HP15_478 0.22 -0.1 488,706 - HP15_478 0.24 +1.3 488,733 - HP15_478 0.26 +1.6 488,857 - HP15_478 0.32 -0.3 488,893 + HP15_478 0.33 -1.9 488,991 + HP15_478 0.38 -0.2 489,281 + HP15_478 0.52 -0.2 489,522 + HP15_478 0.64 +0.5 489,700 + HP15_478 0.72 +1.4 489,829 - HP15_478 0.79 -0.7 489,860 - HP15_478 0.80 -1.0 490,206 - +0.1 490,270 - -0.2 490,384 + +1.7
Or see this region's nucleotide sequence