Experiment: a-Ketoglutaric
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_2147 and Sama_2148 are separated by 104 nucleotides Sama_2148 and Sama_2149 are separated by 12 nucleotides Sama_2149 and truA are separated by 110 nucleotides
Sama_2147: Sama_2147 - colicin V production protein (RefSeq), at 2,594,061 to 2,594,549
_2147
Sama_2148: Sama_2148 - sporulation related (RefSeq), at 2,594,654 to 2,595,325
_2148
Sama_2149: Sama_2149 - FolC bifunctional protein (RefSeq), at 2,595,338 to 2,596,603
_2149
Sama_2150: truA - tRNA pseudouridine synthase A (RefSeq), at 2,596,714 to 2,597,499
truA
Position (kb)
2595
2596
2597 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2594.338 kb on - strand, within Sama_2147 at 2594.349 kb on - strand, within Sama_2147 at 2594.349 kb on - strand, within Sama_2147 at 2594.417 kb on - strand, within Sama_2147 at 2594.499 kb on - strand, within Sama_2147 at 2594.534 kb on - strand at 2596.610 kb on - strand at 2596.634 kb on - strand at 2596.634 kb on - strand at 2596.671 kb on + strand at 2596.672 kb on - strand at 2596.672 kb on - strand at 2596.675 kb on + strand at 2596.903 kb on + strand, within truA at 2596.903 kb on + strand, within truA at 2596.904 kb on - strand, within truA at 2596.920 kb on + strand, within truA at 2596.920 kb on + strand, within truA at 2596.920 kb on + strand, within truA at 2596.920 kb on + strand, within truA at 2596.921 kb on - strand, within truA at 2596.921 kb on - strand, within truA at 2596.960 kb on - strand, within truA at 2596.960 kb on - strand, within truA at 2596.960 kb on - strand, within truA at 2596.960 kb on - strand, within truA at 2596.961 kb on + strand, within truA at 2596.961 kb on + strand, within truA at 2596.961 kb on + strand, within truA at 2596.961 kb on + strand, within truA at 2596.961 kb on + strand, within truA at 2596.961 kb on + strand, within truA at 2596.961 kb on + strand, within truA at 2596.961 kb on + strand, within truA at 2596.961 kb on + strand, within truA at 2596.961 kb on + strand, within truA at 2596.961 kb on + strand, within truA at 2596.962 kb on - strand, within truA at 2596.962 kb on - strand, within truA at 2596.962 kb on - strand, within truA at 2596.962 kb on - strand, within truA at 2596.962 kb on - strand, within truA at 2596.962 kb on - strand, within truA at 2596.962 kb on - strand, within truA at 2596.962 kb on - strand, within truA at 2596.962 kb on - strand, within truA at 2596.989 kb on + strand, within truA at 2596.990 kb on - strand, within truA at 2596.990 kb on - strand, within truA at 2596.990 kb on - strand, within truA at 2596.990 kb on - strand, within truA at 2596.990 kb on - strand, within truA at 2596.990 kb on - strand, within truA at 2596.990 kb on - strand, within truA at 2596.990 kb on - strand, within truA at 2596.993 kb on + strand, within truA at 2596.994 kb on - strand, within truA at 2596.994 kb on - strand, within truA at 2596.994 kb on - strand, within truA at 2597.010 kb on + strand, within truA at 2597.060 kb on - strand, within truA at 2597.060 kb on - strand, within truA at 2597.060 kb on - strand, within truA at 2597.111 kb on + strand, within truA at 2597.163 kb on + strand, within truA at 2597.164 kb on - strand, within truA at 2597.307 kb on + strand, within truA at 2597.339 kb on - strand, within truA at 2597.503 kb on + strand at 2597.504 kb on - strand at 2597.541 kb on + strand at 2597.542 kb on - strand at 2597.544 kb on + strand at 2597.545 kb on - strand at 2597.545 kb on - strand at 2597.599 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric remove 2,594,338 - Sama_2147 0.57 -2.3 2,594,349 - Sama_2147 0.59 -0.3 2,594,349 - Sama_2147 0.59 +1.7 2,594,417 - Sama_2147 0.73 +0.8 2,594,499 - Sama_2147 0.90 +2.3 2,594,534 - -1.9 2,596,610 - -1.8 2,596,634 - -0.3 2,596,634 - +1.7 2,596,671 + -1.2 2,596,672 - +0.1 2,596,672 - +1.0 2,596,675 + -1.7 2,596,903 + truA Sama_2150 0.24 -1.3 2,596,903 + truA Sama_2150 0.24 -2.3 2,596,904 - truA Sama_2150 0.24 +0.2 2,596,920 + truA Sama_2150 0.26 -2.3 2,596,920 + truA Sama_2150 0.26 -1.9 2,596,920 + truA Sama_2150 0.26 -0.9 2,596,920 + truA Sama_2150 0.26 -1.3 2,596,921 - truA Sama_2150 0.26 -0.6 2,596,921 - truA Sama_2150 0.26 -1.3 2,596,960 - truA Sama_2150 0.31 -1.9 2,596,960 - truA Sama_2150 0.31 -1.3 2,596,960 - truA Sama_2150 0.31 -0.6 2,596,960 - truA Sama_2150 0.31 -3.3 2,596,961 + truA Sama_2150 0.31 -0.5 2,596,961 + truA Sama_2150 0.31 -2.7 2,596,961 + truA Sama_2150 0.31 -0.7 2,596,961 + truA Sama_2150 0.31 +0.7 2,596,961 + truA Sama_2150 0.31 -1.8 2,596,961 + truA Sama_2150 0.31 -1.3 2,596,961 + truA Sama_2150 0.31 -1.9 2,596,961 + truA Sama_2150 0.31 -2.5 2,596,961 + truA Sama_2150 0.31 -1.7 2,596,961 + truA Sama_2150 0.31 -1.7 2,596,961 + truA Sama_2150 0.31 -1.1 2,596,962 - truA Sama_2150 0.32 -2.7 2,596,962 - truA Sama_2150 0.32 +0.7 2,596,962 - truA Sama_2150 0.32 -0.9 2,596,962 - truA Sama_2150 0.32 -1.7 2,596,962 - truA Sama_2150 0.32 -0.3 2,596,962 - truA Sama_2150 0.32 -2.1 2,596,962 - truA Sama_2150 0.32 -0.6 2,596,962 - truA Sama_2150 0.32 -1.5 2,596,962 - truA Sama_2150 0.32 -1.1 2,596,989 + truA Sama_2150 0.35 -2.3 2,596,990 - truA Sama_2150 0.35 -1.5 2,596,990 - truA Sama_2150 0.35 -1.3 2,596,990 - truA Sama_2150 0.35 -1.9 2,596,990 - truA Sama_2150 0.35 -1.7 2,596,990 - truA Sama_2150 0.35 -2.7 2,596,990 - truA Sama_2150 0.35 +0.7 2,596,990 - truA Sama_2150 0.35 -3.0 2,596,990 - truA Sama_2150 0.35 -0.2 2,596,993 + truA Sama_2150 0.35 -0.9 2,596,994 - truA Sama_2150 0.36 -0.9 2,596,994 - truA Sama_2150 0.36 -1.3 2,596,994 - truA Sama_2150 0.36 -1.9 2,597,010 + truA Sama_2150 0.38 +1.1 2,597,060 - truA Sama_2150 0.44 -2.9 2,597,060 - truA Sama_2150 0.44 -1.3 2,597,060 - truA Sama_2150 0.44 -1.7 2,597,111 + truA Sama_2150 0.51 -0.9 2,597,163 + truA Sama_2150 0.57 +0.7 2,597,164 - truA Sama_2150 0.57 -2.1 2,597,307 + truA Sama_2150 0.75 -0.9 2,597,339 - truA Sama_2150 0.80 +0.1 2,597,503 + -1.7 2,597,504 - +0.1 2,597,541 + +3.0 2,597,542 - +0.3 2,597,544 + +1.3 2,597,545 - -1.9 2,597,545 - +0.2 2,597,599 - +0.7
Or see this region's nucleotide sequence