Experiment: Vancomycin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3478 and rpmG are separated by 323 nucleotides rpmG and Shew_3480 are separated by 6 nucleotides Shew_3480 and Shew_3481 are separated by 123 nucleotides Shew_3481 and Shew_3482 are separated by 111 nucleotides
Shew_3478: Shew_3478 - N-acetylglutamate synthase (RefSeq), at 4,164,531 to 4,165,853
_3478
Shew_3479: rpmG - 50S ribosomal protein L33 (RefSeq), at 4,166,177 to 4,166,350
rpmG
Shew_3480: Shew_3480 - ribosomal protein L28 (RefSeq), at 4,166,357 to 4,166,593
_3480
Shew_3481: Shew_3481 - DNA repair protein RadC (RefSeq), at 4,166,717 to 4,167,418
_3481
Shew_3482: Shew_3482 - phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase (RefSeq), at 4,167,530 to 4,168,726
_3482
Position (kb)
4166
4167
4168 Strain fitness (log2 ratio)
-1
0
1 at 4165.814 kb on - strand at 4166.022 kb on + strand at 4166.056 kb on + strand at 4166.056 kb on + strand at 4166.073 kb on - strand at 4166.983 kb on + strand, within Shew_3481 at 4166.993 kb on + strand, within Shew_3481 at 4166.995 kb on + strand, within Shew_3481 at 4167.017 kb on + strand, within Shew_3481 at 4167.215 kb on - strand, within Shew_3481 at 4167.331 kb on - strand, within Shew_3481 at 4167.408 kb on - strand at 4167.432 kb on - strand at 4167.488 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.0625 mg/ml remove 4,165,814 - +0.4 4,166,022 + +0.4 4,166,056 + -0.1 4,166,056 + -0.3 4,166,073 - -0.5 4,166,983 + Shew_3481 0.38 +0.7 4,166,993 + Shew_3481 0.39 +0.1 4,166,995 + Shew_3481 0.40 +0.3 4,167,017 + Shew_3481 0.43 +1.0 4,167,215 - Shew_3481 0.71 -0.3 4,167,331 - Shew_3481 0.87 +0.3 4,167,408 - +0.3 4,167,432 - +0.1 4,167,488 + +0.0
Or see this region's nucleotide sequence